Setting up and monitoring an infection of Dictyostelium discoideum with mycobacteria.
Setting up and monitoring an infection of Dictyostelium discoideum with mycobacteria.
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DOI：
10.1007/978-1-62703-302-2_22
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发表时间：
2013-01-01
期刊：
影响因子：
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通讯作者：
Soldati, Thierry
中科院分区：
文献类型：
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作者：
Arafah, Sonia;Kicka, Sebastien;Soldati, Thierry
海分枝杆菌是野生鱼类和两栖动物结核病的病原体。它是结核分枝杆菌的遗传近亲，因此感染过程在细胞和有机体水平上与人类结核分枝杆菌感染的许多特征非常相似。因此，海分枝杆菌被用作研究各种宿主生物体中分枝杆菌感染的模型。最近，盘基网柄菌-M。 marinum 系统已被证明是一个有价值的模型，它概括了 M. marinum 细胞内命运的主要特征，包括吞噬体成熟停滞，以及其特定的细胞间传播模式。我们在这里提出了一个包含详细方法的“入门套件”，允许用海分枝杆菌建立盘基网柄菌感染并定量监测细胞内细菌的生长。
Mycobacterium marinum is the causative agent of fish and amphibian tuberculosis in the wild. It is a genetically close cousin of Mycobacterium tuberculosis, and thereby the infection process remarkably shares many of the hallmarks of M. tuberculosis infection in human, at both the cellular and organism levels. Therefore, M. marinum is used as a model for the study of mycobacterial infection in various host organisms. Recently, the Dictyostelium-M. marinum system has been shown to be a valuable model that recapitulates the main features of the intracellular fate of M. marinum including phagosome maturation arrest, as well as its particular cell-to-cell dissemination mode. We present here a "starter kit" of detailed methods that allows to establish an infection of Dictyostelium with M. marinum and to monitor quantitatively the intracellular bacterial growth.