Electron paramagnetic resonance oxygen images correlate spatially and quantitatively with oxylite oxygen measurements
Electron paramagnetic resonance oxygen images correlate spatially and quantitatively with oxylite oxygen measurements
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DOI：
10.1158/1078-0432.ccr-05-0446
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发表时间：
2006-07-15
影响因子：
11.5
通讯作者：
Halpern, Howard J.
中科院分区：
文献类型：
--
作者：
Elas, Martyna;Ahn, Kang-Hyun;Halpern, Howard J.
肿瘤氧合预测癌症治疗反应和恶性表型。这就产生了许多氧含量测定法。不同血氧测定法的比较对于验证和理解这些技术至关重要，电子顺磁共振（EPR）成像是一种用于提供肿瘤和组织氧合定量高分辨率图像的新技术。这项工作比较了肿瘤pO（2）值序列从EPR氧图像与序列的氧测量沿沿着一个轨道与Oxylite氧探头。四维（三个空间和一个光谱）EPR氧图像使用光谱成像技术来测量来自自旋探针注射后小鼠组织和肿瘤中的三苯甲基自旋探针（OX063，阿默舍姆Health R&D）的每个图像体素中的光谱线的宽度。简单的校准允许将每个线宽直接定量转换为氧浓度。这些四维EPR图像，在45分钟内从C3H小鼠腿部生长的FSa纤维肉瘤获得，具有类似于1 mm的空间分辨率和类似于3 Torr的氧分辨率。使用定制的立体定向定位装置测量Oxylite轨迹的位置，精度在2 mm以内。共获得9张图像，涉及17个轨道。其中，大多数显示出Oxylite测量的pO（2）和位于肿瘤中的轨迹之间的良好相关性，在Oxylite定位的不确定性范围内。在空间分布模式和pO（2）幅度方面，相关性良好。这两种模式的强相关性证实了EPR成像作为研究肿瘤氧合的有用工具。
Tumor oxygenation predicts cancer therapy response and malignant phenotype. This has spawned a number of oxymetries. Comparison of different oxymetries is crucial for the validation and understanding of these techniques, Electron paramagnetic resonance (EPR) imaging is a novel technique for providing quantitative high-resolution images of tumor and tissue oxygenation. This work compares sequences of tumor pO(2) values from EPR oxygen images with sequences of oxygen measurements made along a track with an Oxylite oxygen probe. Four-dimensional (three spatial and one spectral) EPR oxygen images used spectroscopic imaging techniques to measure the width of a spectral line in each image voxel from a trityl spin probe (OX063, Amersham Health R&D) in the tissues and tumor of mice after spin probe injection. A simple calibration allows direct, quantitative translation of each line width to an oxygen concentration. These four-dimensional EPR images, obtained in 45 minutes from FSa fibrosarcomas grown in the legs of C3H mice, have a spatial resolution of similar to 1 mm and oxygen resolution of similar to 3 Torr. The position of the Oxylite track was measured within a 2-mm accuracy using a custom stereotactic positioning device. A total of nine images that involve 17 tracks were obtained. Of these, most showed good correlation between the Oxylite measured pO(2) and a track located in the tumor within the uncertainties of the Oxylite localizability. The correlation was good both in terms of spatial distribution pattern and pO(2) magnitude. The strong correlation of the two modalities corroborates EPR imaging as a useful tool for the study of tumor oxygenation.