Improving efficiency and reducing enzyme inactivation during lipase-mediated epoxidation of α-pinene in a double-phase reaction system
Improving efficiency and reducing enzyme inactivation during lipase-mediated epoxidation of α-pinene in a double-phase reaction system
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DOI：
10.1007/s00449-023-02902-4
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发表时间：
2023-07
影响因子：
3.8
通讯作者：
Lishuang Yu;Cheng Zou;Qingyun Li;Zhaoming Liu;Youyan Liu;Aixing Tang
中科院分区：
文献类型：
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作者：
Lishuang Yu;Cheng Zou;Qingyun Li;Zhaoming Liu;Youyan Liu;Aixing Tang
脂肪酶催化的烯烃化学酶环氧化是一种绿色环保的替代工艺。然而，传统的有机-水两相反应体系所造成的传质障碍和脂肪酶失活问题一直是研究者关注的焦点。为了克服这些问题，我们考察了反应温度和两种重要底物(双氧水和酰基供体)对环氧化反应和界面传质的影响。确定了最佳反应条件：温度30℃，以30wt-%H_2O_2为氧源，以1M月桂酸为氧气载体。此外，通过模拟摇瓶反应条件，设计了间歇反应器，并增加了金属网，有效地阻断了高浓度过氧化氢与酶的直接接触。在最佳反应条件下，环氧化反应5h，产品收率最高可达93.2%。此外，经过7次重复实验，该脂肪酶仍保持51.2%的相对活性。
Chemoenzymatic epoxidation of olefin mediated by lipase is a green and environmentally friendly alternative process. However, the mass transfer barrier and lipase deactivation caused by the traditional organic–water biphasic reaction system have always been the focus of researchers’ attention. To overcome these issues, we investigated the effects of reaction temperature and two important substrates (H2O2and acyl donor) on the epoxidation reaction and interfacial mass transfer. As a result, we determined the optimal reaction conditions: a temperature of 30 °C, 30 wt-% H2O2as the oxygen source, and 1 M lauric acid as the oxygen carrier. Additionally, by simulating the conditions of shaking flask reactions, we designed a batch reactor and added a metal mesh to effectively block the direct contact between high-concentration hydrogen peroxide and the enzyme. Under these optimal conditions, the epoxidation reaction was carried out for 5 h, and the product yield reached a maximum of 93.2%. Furthermore, after seven repetitive experiments, the lipase still maintained a relative activity of 51.2%.