An efficient method for the collection of root mucilage from different plant species : A case study on the effect of mucilage on soil water repellency
An efficient method for the collection of root mucilage from different plant species : A case study on the effect of mucilage on soil water repellency
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DOI：
10.1002/jpln.201500511
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发表时间：
2016-04
影响因子：
2.5
通讯作者：
I. Zickenrott;S. Woche;J. Bachmann;M. Ahmed;D. Vetterlein
中科院分区：
文献类型：
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作者：
I. Zickenrott;S. Woche;J. Bachmann;M. Ahmed;D. Vetterlein
根粘液可能在理解根吸水方面发挥突出作用，因此，人们对研究根粘液的功能重新产生了兴趣。然而，粘液的研究受到粘液收集的繁琐程序的阻碍。我们开发了一种粘液分离器，其利用低离心力（570 rpm）将粘液与种子根分离，而不需要处理单个种子或将发芽的种子从托盘/筛网移到离心管。对于不同的植物物种，可以产生1至3.7 mL托盘-1的水合粘液，在45 min内连续处理6个托盘。对于小麦，其显示干物质含量为0.5%，这相当于98.6 mg粘液干物质。玉米的总产量最低，只有34毫克干物质。标准化根尖产生的粘液量与文献数据吻合良好。通过新方法获得的粘液被用来测量其对土壤的排斥性的影响，因为这种性质直接与再润湿过程中根际土壤含水量较低的现象有关。结果表明，根际粘液的数量和质量对根际植物的驱避作用有很大的影响。在测试的物种（羽扇豆，蚕豆，玉米，小麦），最大的差异之间观察到两种豆类。对于玉米种子根粘液获得与新系统进行了比较，空气出生的支撑根粘液。这两种代表不同根目的粘液之间的差异清楚地表明，仍然需要能够研究老植物根的方法。
Root mucilage may play a prominent role in understanding root water uptake and, thus, there is revived interest in studying the function of root mucilage. However, mucilage research is hampered by the tedious procedures of mucilage collection. We developed a mucilage separator which utilizes low centrifugal forces (570 rpm) to separate the mucilage from seminal roots without the need of handling individual seeds or removing the germinated seeds from the tray/mesh to a centrifuge tube. For the different plant species, between 1 and 3.7 mL tray−1 of hydrated mucilage could be produced, with 6 trays being handled successively within 45 min. For Triticum aestivum, which showed a dry matter content of 0.5%, this was equivalent to 98.6 mg mucilage dry matter. The lowest total production was found for Zea mays with just 34 mg dry matter. The amounts of mucilage produced normalized to root tip agree well with literature data. The mucilage obtained by the new method was used to measure its effect on repellency of soil as this property directly relates to the phenomenon of lower rhizosphere soil water content during rewetting. It could be shown that repellency of the rhizosphere is affected by the quantity as well as by species-dependent quality of mucilage in the rhizosphere. Among the species tested (Lupinus albus, Vicia faba, Zea mays, Triticum aestivum), the largest differences were observed between the two legumes. For Zea mays seminal root mucilage obtained with the new system was compared to mucilage of air born brace roots. The differences between these two mucilages, representing different root orders, indicate clearly that there is still a need for methods which enable the investigation of roots from older plants.