Triosephosphate isomerase and peroxiredoxin 6, two novel serum markers for human lung squamous cell carcinoma
Triosephosphate isomerase and peroxiredoxin 6, two novel serum markers for human lung squamous cell carcinoma
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DOI：
10.1111/j.1349-7006.2009.01314.x
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发表时间：
2009-12-01
期刊：
影响因子：
5.7
通讯作者：
Chen, Zhu-chu
中科院分区：
文献类型：
--
作者：
Zhang, Xiu-zhi;Xiao, Zhe-feng;Chen, Zhu-chu
目前对鉴定用于癌症的诊断和免疫治疗的人肿瘤抗原有很大的兴趣。在我们以前的研究中，观察到磷酸丙糖异构酶在肺鳞癌中的分泌特征和上调，并且在超过25%的患者血清中检测到磷酸丙糖异构酶和过氧化物酶6的自身抗体，而在健康对照中没有检测到。在这项研究中，在患者血清中也发现了较高水平的过氧化物酶6。通过酶联免疫吸附试验，在另外61例肺鳞状细胞癌患者、23例肺腺癌患者、56例其他类型的癌症患者、12例良性肺病患者和59例健康对照中进一步验证了磷酸丙糖异构酶和过氧化物氧还蛋白6的上调。我们发现，磷酸丙糖异构酶和peroxiredoxin 6在肺鳞状细胞癌血清中特异性升高，与其他组相比，肺腺癌患者的peroxiredoxin 6除外。磷酸丙糖异构酶与肺鳞癌远处转移呈正相关，在受试者工作特征曲线上以0.221（光密度值）为分界点时，磷酸丙糖异构酶对肺鳞癌和健康对照者的鉴别诊断敏感性为65.6%，特异性为84.7%，总准确率为75%。Peroxiredoxin 6在0.151的临界点，它可以区分两组的敏感性为70.5%，特异性为62.7%，总的准确性为65.8%。用磷酸丙糖异构酶和过氧化物氧还蛋白6进行判别分析，结果表明，68.9%的肺鳞癌和83.1%的健康对照的正确分类。我们认为磷酸丙糖异构酶和过氧化物氧还蛋白6可以作为肺鳞癌的标志物。（Cancer Sci 2009; 100：2396-2401）。
There is currently substantial interest in the identification of human tumor antigens for the diagnosis and immunotherapy of cancer. In our previous study, secretion character and up-regulation of triosephosphate isomerase were observed in lung squamous cell carcinoma, and autoantibodies against triosephosphate isomerase and peroxiredoxin 6 were detected in the sera from over 25% of patients, but in none of the healthy controls. In this study, peroxiredoxin 6 was also found at higher levels in the sera of the patients. Up-regulated triosephosphate isomerase and peroxiredoxin 6 were further validated by enzyme-linked immunosorbent assay in an additional 61 lung squamous cell carcinoma patients, 23 lung adenocarcinoma patients, 56 other types of carcinoma patients, 12 benign lung disease patients, and 59 healthy controls. We found that both triosephosphate isomerase and peroxiredoxin 6 were specifically elevated in lung squamous cell carcinoma sera compared with other groups, with the exception of peroxiredoxin 6 in lung adenocarcinoma patients. Positive correlation between triosephosphate isomerase and distant metastasis was found. At the cut-off point 0.221 (optical density value) on the receiver operating characteristic curve, triosephosphate isomerase could comparatively discriminate lung squamous cell carcinoma from healthy controls with a sensitivity of 65.6%, specificity 84.7%, and total accuracy 75%. For peroxiredoxin 6, at the cut-off point 0.151, it could discriminate the two groups with a sensitivity of 70.5%, specificity 62.7%, and total accuracy 65.8%. With both triosephosphate isomerase and peroxiredoxin 6, discriminant analysis results showed that 68.9% of the lung squamous cell carcinoma and 83.1% of healthy controls were correctly classified. We concluded that triosephosphate isomerase and peroxiredoxin 6 could be markers for lung squamous cell carcinoma. (Cancer Sci 2009; 100: 2396-2401).