Chronic low-dose administration of nicotine facilitates recovery and synaptic change after focal ischemia in rats
Chronic low-dose administration of nicotine facilitates recovery and synaptic change after focal ischemia in rats
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DOI：
10.1016/j.neuropharm.2005.11.018
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发表时间：
2006-06-01
影响因子：
4.7
通讯作者：
Kolb, Bryan
中科院分区：
文献类型：
--
作者：
Gonzalez, Claudia L. R.;Gharbawie, Omar A.;Kolb, Bryan
目前的研究探讨了长期给予尼古丁对大鼠局灶性中风后运动行为的影响。动物在托盘到达任务中训练2周，然后将它们分为：（1）对照+盐水（2）对照+尼古丁（3）中风+盐水，和（4）中风+尼古丁组。通过对用于伸手的前爪对侧的感觉运动皮质的表面血管进行去血管术来产生损伤。损伤后48小时，动物每天接受两次尼古丁（0.3 mg/kg）或生理盐水（0.9%）注射，共持续12天。在损伤后1周和每隔一周在运动电池中测试动物，共7周。前肢和扣带区的锥体细胞，然后检查树突的长度和分支使用Golgi-Cox程序。行为结果表明，在测试结束时，中风+尼古丁动物相对于中风+盐水动物显示出显著的行为改善。中风+尼古丁动物显示前肢和扣带区锥体细胞的树突长度和分支增加。结果表明，中风+尼古丁组的行为增强可能归因于残余皮质运动区的树突生长增强。(c)2005爱思唯尔有限公司保留所有权利。
The current study examines the effects of chronic administration of nicotine on motor behavior after focal stroke in rats. Animals were trained in a tray-reaching task for 2 weeks and then they were divided into: (1) control + saline (2) control + nicotine (3) stroke + saline, and (4) stroke + nicotine groups. Lesions were produced by devascularization of the surface blood vessels of the sensorimotor cortex contralateral to the forepaw used for reaching. Forty-eight hours after the lesions, and for a total of 12 days, animals received two daily injections of either nicotine (0.3 mg/kg) or saline (0.9%). Animals were tested in a motor battery I week after the lesions and every other week for a total of 7 weeks. Pyramidal cells in forelimb and cingulate areas were then examined for dendritic length and branching using a Golgi-Cox procedure. Behavioral results demonstrated that by the end of the testing stroke + nicotine animals showed significant behavioral improvement relative to stroke + saline animals. Stroke + nicotine animals showed an increase in dendritic length and branching in pyramidal cells of the forelimb and cingulate areas. The results suggest that the behavioral enhancement in the stroke + nicotine group might be attributable to the enhanced dendritic growth in residual cortical motor regions. (c) 2005 Elsevier Ltd. All rights reserved.