Neurotoxin-conjugated upconversion nanoprobes for direct visualization of tumors under near-infrared irradiation.
Neurotoxin-conjugated upconversion nanoprobes for direct visualization of tumors under near-infrared irradiation.
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DOI：
10.1016/j.biomaterials.2010.07.099
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发表时间：
2010-11
期刊：
影响因子：
14
通讯作者：
Xue-feng Yu;Z. Sun;Min Li;Yang Xiang;Ququan Wang;Fenfen Tang;Yingliang Wu;Zhijian Cao;Wenxin Li
中科院分区：
文献类型：
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作者：
Xue-feng Yu;Z. Sun;Min Li;Yang Xiang;Ququan Wang;Fenfen Tang;Yingliang Wu;Zhijian Cao;Wenxin Li
我们报告了神经毒素介导的上转换纳米探针在活体动物中的肿瘤靶向和可视化的发展。通过制备聚乙烯亚胺涂覆的六方相NaYF 4：Yb，Er/Ce纳米颗粒并将其与重组氯毒素缀合来合成纳米探针，所述重组氯毒素是一种典型的肽神经毒素，其可以高度特异性地结合多种类型的癌细胞。通过激光扫描上转换荧光显微镜观察特异性靶向胶质瘤细胞的纳米探针。通过细胞和动物毒性测定显示良好的探针生物相容性。使用静脉内注射纳米探针的Balb-c裸鼠进行动物研究。所获得的高对比度图像显示出高度特异性的肿瘤结合和在980 nm近红外照射下具有明亮的红色荧光的直接肿瘤可视化。神经毒素介导的上转换纳米探针的高灵敏度和高特异性以及用于肿瘤可视化的所需光学装置的简化表明了一种可能有助于提高肿瘤患者可用的诊断和治疗方式的有效性的方法。
We report the development of neurotoxin-mediated upconversion nanoprobes for tumor targeting and visualization in living animals. The nanoprobes were synthesized by preparing polyethylenimine-coated hexagonal-phase NaYF4:Yb,Er/Ce nanoparticles and conjugating them with recombinant chlorotoxin, a typical peptide neurotoxin that could bind with high specificity to many types of cancer cells. Nanoprobes that specifically targeted glioma cells were visualized by laser scanning upconversion fluorescence microscopy. Good probe biocompatibility was displayed with cellular and animal toxicity determinations. Animal studies were performed using Balb-c nude mice injected intravenously with the nanoprobes. The obtained high-contrast images demonstrated highly specific tumor binding and direct tumor visualization with bright red fluorescence under 980-nm near-infrared irradiation. The high sensitivity and high specificity of the neurotoxin-mediated upconversion nanoprobes and the simplification of the required optical device for tumor visualization suggest an approach that may help improve the effectiveness of the diagnostic and therapeutic modalities available for tumor patients.