Insights into Roseburia intestinalis which alleviates experimental colitis pathology by inducing anti-inflammatory responses
Insights into Roseburia intestinalis which alleviates experimental colitis pathology by inducing anti-inflammatory responses
复制标题
深入了解肠罗斯伯利亚。
DOI：
10.1111/jgh.14144
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发表时间：
2018-10-01
影响因子：
4.1
通讯作者：
Wang, Xiaoyan
中科院分区：
文献类型：
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作者：
Shen, Zhaohua;Zhu, Changxin;Wang, Xiaoyan
背景与目的本研究旨在探讨Roseburia ninteinalis （r.e ninteinalis）对克罗恩病（CD）的抗炎作用及其机制。方法采用16s - rrna基因组测序技术检测未经治疗的乳糜泻患者和健康对照者肠道菌群特征。然后，采用TNBS结肠炎模型，研究rninteinalis对疾病活动指数评分、肠道病理、Treg细胞分化、胸腺基质淋巴生成素（TSLP）、TGF-、IL-10表达的影响。在细胞水平上，本研究采用LPS刺激Caco-2细胞进行炎症模型，然后与r.n testinalis共培养，检测TSLP和TGF-的变化。然后，本研究用肠草刺激外周血单个核细胞，检测Treg细胞的变化。结果未经治疗的CD患者（n=10）粪便样本的基因组测序显示，肠道微生物群的丰度和多样性下降，包括肠球菌。此外，在结肠炎小鼠模型中，rninteinalis可降低疾病活动指数评分、结肠缩短、肠黏膜上皮损伤和粘膜淋巴细胞浸润。它通过增加Treg细胞数量和抗炎细胞因子TSLP、TGF-和白细胞介素-10的表达来抑制肠道炎症(P
Background and AimThe study aims to elucidate the anti-inflammatory effect and mechanism of Roseburia intestinalis (R.intestinalis) in Crohn's disease (CD).Methods16S-rRNA genome sequencing technique is used to detect the characteristics of intestinal microbiota in untreated CD patients and healthy controls. Then the study investigates the effects of R.intestinalis on disease activity index score, intestinal pathology, the differentiation of Treg cells, and the expressions of Thymic stromal lymphopoietin (TSLP), TGF- and IL-10 by using TNBS colitis models. At the cellular level, the study uses LPS to stimulate Caco-2 cells to conduct inflammation models and then co-culture with R.intestinalis and detect changes of TSLP and TGF-. The study then uses R.intestinalis to stimulate peripheral blood mononuclear cells, and the change of Treg cells was detected.ResultsGenome sequencing of fecal samples from untreated CD patients (n=10) revealed decreases in the abundance and diversity of intestinal microbiota, including R.intestinalis. Moreover, R.intestinalis reduced disease activity index scores, colon shortening, intestinal mucosal epithelial injury, and mucosal lymphocyte infiltration in a colitis mice model. It suppressed intestinal inflammation by increasing Treg cell numbers and expression of the anti-inflammatory cytokines TSLP, TGF-, and interleukin-10 (P