EVIDENCE SUGGESTING THAT L-FUCOSE IS PART OF A RECOGNITION SIGNAL FOR SPERM-ZONA PELLUCIDA ATTACHMENT IN MAMMALS
EVIDENCE SUGGESTING THAT L-FUCOSE IS PART OF A RECOGNITION SIGNAL FOR SPERM-ZONA PELLUCIDA ATTACHMENT IN MAMMALS
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DOI：
10.1002/mrd.1120050406
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
YANAGIMACHI, R
中科院分区：
文献类型：
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作者：
HUANG, TTF;OHZU, E;YANAGIMACHI, R
本实验旨在研究单糖和复合多糖对哺乳动物精子与透明带附着的影响。在豚鼠体内，与其他糖相比，L- focal在50 mM时的附着抑制剂效果好2倍。富含硫酸化L- focal的藻脂糖苷是一种非常有效的抑制剂，在浓度为100 . μ g/ml时完全阻断附着。其他几种高硫酸盐化的糖胺聚糖没有表现出抑制活性，这表明岩藻蛋白的作用不仅仅是由于其电荷或硫酸盐。此外，部分水解产生的岩藻糖蛋白片段，用生物凝胶P-2分离，在重量基础上几乎与天然分子一样具有抑制作用。岩藻素还能抑制仓鼠和人的精带附着；它的作用不是特定于物种的。这些数据表明L-聚焦可能是哺乳动物配子之间识别信号的一部分。
Experiments were designed to test the effects of simple sugars and complex polysaccharides on the attachment of mammalian spermatozoa with the zona pellucida. In the guinea pig, L-fucose was a 2-fold better inhibitor of the attachment compared to other sugars at 50 mM. Fucoidin, an algal polysaccharide rich in sulfated L-fucose, was a very potent inhibitor, completely blocking attachment at a concentration of 100 .mu.g/ml. Several other highly sulfated glycosaminoglycans showed no inhibitory activity, suggesting the fucoidin effect was not simply due to its charge or sulfate. In addition, fragments of fucoidin, generated by partial hydrolysis and isolated using biogel P-2, were nearly as inhibitory as the native molecule on a weight basis. Fucoidin also inhibited sperma-zona attachment in the hamster and human; its effect is not species specific. The data suggest that L-fucose may be part of a recognition signal between mammalian gametes.