Enriched gonadal migration of donor-derived gonadal primordial germ cells by immunomagnetic cell sorting in birds
Enriched gonadal migration of donor-derived gonadal primordial germ cells by immunomagnetic cell sorting in birds
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DOI：
10.1002/mrd.20051
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发表时间：
2004-05-01
影响因子：
2.5
通讯作者：
Han, JY
中科院分区：
文献类型：
--
作者：
Kim, JN;Kim, MA;Han, JY
本研究旨在探讨免疫磁处理是否能提高5.5日龄雏鸡和5日龄鹌鹑生殖腺原始生殖细胞（gPGCs）的回收和迁移能力。将收集到的gPGC与特异性gPGC抗体结合后，装入磁激活细胞分选器（MACS）中，随后将每个物种中经MACS处理或未处理的细胞转移到受体胚胎中。MACS处理显著（P < 0.05）提高了所得性腺细胞中gPGCs的群体比例（鸡0.74 ~ 33.4%，鹌鹑2.68 ~ 45.1%）。这是由于总细胞群中非gpgcs的数量减少。MACS处理进一步增强了这两个物种转移的gPGCs的性腺迁移（在鸡中为10%对80-85%，在鹌鹑中为10-15%对70-80%）。微注射细胞数量增加到600个细胞/胚胎，并没有消除这种促进作用。综上所述，MACS处理显著提高了禽类gPGCs在性腺细胞中的种群比例，促进了受体胚胎的性腺迁移。(C) 2004 Wiley-Liss, Inc。
This study was conducted to evaluate whether immunomagnetic treatment could improve the retrieval and migration capacity of avian gonadal primordial germ cells (gPGCs) collected from gonads in 5.5-day-old chick and 5-day-old quail embryos, respectively. Collected gPGCs were loaded into a magnetic-activated cell sorter (MACS) after being conjugated with specific gPGC antibodies and either MACS-treated or non-treated cells in each species were subsequently transferred to the recipient embryos. MACS treatment significantly (P < 0.05) increased the population ratio of gPGCs in gonadal cells retrieved (0.74 to 33.4% in the chicken and 2.68 to 45.1% in the quail). This was due to decreased number of non-gPGCs in total cell population. MACS treatment further enhanced gonadal migration of gPGCs transferred in both species (10% vs. 80-85% in the chicken and 10-15% vs. 70-80% in the quail). Increase in the number of microinjected cells up to 600 cells/embryo did not eliminate such promoting effect. In conclusion, MACS treatment greatly increased the population ratio of avian gPGCs in gonadal cells, resulting improved gonadal migration in recipient embryos. (C) 2004 Wiley-Liss, Inc.