Epidemics of GI.2 sapovirus in gastroenteritis outbreaks during 2012-2013 in Osaka City, Japan
Epidemics of GI.2 sapovirus in gastroenteritis outbreaks during 2012-2013 in Osaka City, Japan
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DOI：
10.1002/jmv.24451
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发表时间：
2016-07-01
影响因子：
12.7
通讯作者：
Kaida, Atsushi
中科院分区：
文献类型：
--
作者：
Iritani, Nobuhiro;Yamamoto, Seiji P.;Kaida, Atsushi
Sapovirus（SaV）是在散发病例和爆发中引起人类胃肠炎的病原体。在2005年1月至2014年8月期间，使用实时RT-PCR在日本大坂市的510起胃肠炎疫情中检测到30起（5.9%）SaV。SaV相关疫情的季节性分布显示，2011-2012年期间疫情有所增加，2012-2013年期间疫情频率最高。基于衣壳区的基因分型结果表明，最常见的基因型为GI. 2（36. 7%），其中菌株的亲缘关系密切。18株GI.2型肠球菌衣壳蛋白基因全序列的比较1990年至2013年期间（本研究中的7个菌株和GenBank中的11个菌株）显示GI. 2菌株至少可分为三个遗传簇（1990-2000，2004-2007，和2008-2013），在预测的P结构域中具有时间上独特的氨基酸残基和突变的积累，这可能是GI. 2菌株出现和传播的原因之一。本研究也将有助于理解SaV基因组的进化机制。J. Med. Virol. 88：1187-1193，2016. (c)2015 Wiley Periodicals，Inc.
Sapovirus (SaV) is a causative agent of gastroenteritis in humans in both sporadic cases and outbreaks. During the period from January 2005 to August 2014, SaV was detected in 30 (5.9%) of 510 gastroenteritis outbreaks in Osaka City, Japan using real-time RT-PCR. Seasonal distribution of SaV-associated outbreaks revealed an increase during the 2011-2012 season and the highest frequency of outbreaks during the 2012-2013 season. Genotyping analysis based on the capsid region demonstrated that the most common genotype was GI.2 (36.7%), in which the strains were closely related. The comparison of complete capsid gene sequences with 18 GI.2 strains (7 strains in this study and 11 from GenBank) between 1990 and 2013 showed that GI.2 strains were classified into at least three genetic clusters (1990-2000, 2004-2007, and 2008-2013) with chronologically unique amino acid residues and accumulation of mutations in the predicted P domain, suggesting the one of the causes of emergence and spread of GI.2 strains. This study will also be helpful for understanding the evolutionary mechanism of the SaV genome. J. Med. Virol. 88:1187-1193, 2016. (c) 2015 Wiley Periodicals, Inc.