Rational Design of a Two-Photon Fluorogenic Probe for Visualizing Monoamine Oxidase A Activity in Human Glioma Tissues
Rational Design of a Two-Photon Fluorogenic Probe for Visualizing Monoamine Oxidase A Activity in Human Glioma Tissues
复制标题
用于可视化人胶质瘤组织中单胺氧化酶 A 活性的双光子荧光探针的合理设计
DOI：
10.1002/anie.202000059
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发表时间：
2020-05-04
影响因子：
16.6
通讯作者：
Huang, Wei
中科院分区：
文献类型：
--
作者：
Fang, Haixiao;Zhang, Hang;Huang, Wei
单胺氧化酶有两种功能不同但结构相似的同工型（MAO-A和MAO-B）。通过使用荧光检测/成像技术来区分它们的能力具有显著的生物相关性，但对于可用的化学工具来说具有高度挑战性。在此，我们报告了第一个MAO-A特异性双光子荧光探针（F1），能够选择性成像内源性MAO-A酶活性从各种生物样品，包括MAO-A表达神经元SY-SY 5 Y细胞，肿瘤小鼠的大脑和人类胶质瘤组织通过使用双光子荧光显微镜（TPFM）以最小的细胞毒性。
Monoamine oxidases have two functionally distinct but structurally similar isoforms (MAO-A and MAO-B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO-A-specific two-photon fluorogenic probe (F1), capable of selective imaging of endogenous MAO-A enzymatic activities from a variety of biological samples, including MAO-A-expressing neuronal SY-SY5Y cells, the brain of tumor-bearing mice and human Glioma tissues by using two-photon fluorescence microscopy (TPFM) with minimal cytotoxicity.