Dissection of the role of paeoniflorin in the traditional Chinese medicinal formula Si-Ni-San against contact dermatitis in mice
Dissection of the role of paeoniflorin in the traditional Chinese medicinal formula Si-Ni-San against contact dermatitis in mice
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中药方四逆散中芍药苷对小鼠接触性皮炎作用的剖析。
DOI：
10.1016/j.lfs.2008.12.023
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发表时间：
2009-03-13
期刊：
影响因子：
6.1
通讯作者：
Xu, Qiang
中科院分区：
文献类型：
--
作者：
Sun, Yang;Dong, Yi;Xu, Qiang
目的：中药方剂中特定活性成分的作用尚未明确。本研究选择性地对中药四逆散中芍药苷进行缺失和补充，旨在明确芍药苷发挥作用的分子基础。主要方法：采用苦氯诱发小鼠接触性皮炎。通过免疫亲和柱选择性地从四逆散中去除芍药苷。结果：四逆散和芍药苷均能显著降低小鼠耳肿胀度，而芍药苷缺失的四逆散（PF-）对小鼠耳肿胀度无明显改善作用。在脂多糖中。诱发的巨噬细胞、四逆散和芍药苷显著抑制肿瘤坏死因子-α的产生、环氧合酶-2的活性以及细胞外信号调节激酶1/2的磷酸化，而四逆散（PF-）没有或轻微抑制作用。此外，在四逆散（PF-）中补充不同比例的芍药苷，对肿瘤坏死因子-α产生的抑制作用再次出现。此外，在T细胞，而不是巨噬细胞的巨噬细胞移动抑制因子的表达，明显抑制四逆散，但不四逆散（PF-）。我们的数据表明，芍药苷是主要成分的四逆散，发挥负调节巨噬细胞的功能在接触性dermatitis.Significance：本研究表明，解剖的作用，具体成分的药物配方，通过选择性删除和补充，可能是一个有用的策略，在认识和验证的活性成分在传统中药。(C)2009 Elsevier Inc. All rights reserved.
Aims: The roles of specific active ingredients in Chinese medicinal formulas have not been clearly elucidated. In this Study, we selectively deleted and replenished paeoniflorin from Si-Ni-San, a traditional Chinese prescription, and aimed to identify the molecular basis of how paeoniflorin exerted its effect in Si-Ni-San.Main methods: Contact dermatitis was induced in mice with picryl chloride. Paeoniflorin was selectively deleted from Si-Ni-San by an immunoaffinity column. Quantitative real-time PCR, western blot, and enzyme-linked immunosorbent assay were used in this study.Key findings: Both Si-Ni-San and paeoniflorin significantly reduced ear swelling in mice while the paeoniflorin-deleted Si-Ni-San (Si-Ni-San(PF-)) showed little ameliorative effect. In lipopolysaccharide. evoked macrophages, Si-Ni-San and paeoniflorin markedly inhibited tumor necrosis factor-alpha production, cyclooxygenase-2 activity, as well as extracellular signal-regulated kinase 1/2 phosphorylation while Si-Ni-San(PF-) exhibited no or slight inhibitory effect. Furthermore, the inhibitory effect on the production of tumor necrosis factor-alpha reappeared when different proportions of paeoniflorin were replenished in Si-Ni-San(PF-). In addition, the expression of macrophage migration inhibitory factor in T cells, rather than macrophages, was significantly inhibited by Si-Ni-San, but not Si-Ni-San(PF-). Our data indicate paeoniflorin is the principal component of Si-Ni-San, exerting negative regulation on the function of macrophages in contact dermatitis.Significance: The present study suggests that dissecting the role of specific constituents in medicinal formulas through selective deletion and replenishment may be a useful strategy in recognizing and validating an active ingredient in traditional Chinese medicine. (C) 2009 Elsevier Inc. All rights reserved.