EVIDENCE OF ESTROGEN-RECEPTORS IN NORMAL HUMAN OSTEOBLAST-LIKE CELLS
EVIDENCE OF ESTROGEN-RECEPTORS IN NORMAL HUMAN OSTEOBLAST-LIKE CELLS
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DOI：
10.1126/science.3388021
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发表时间：
1988-07-01
期刊：
影响因子：
56.9
通讯作者：
RIGGS, BL
中科院分区：
文献类型：
--
作者：
ERIKSEN, EF;COLVARD, DS;RIGGS, BL
在七种培养的正常人类成骨细胞样细胞中，平均每个细胞核中有1615个氚标记的17β-雌二醇分子可以结合到特定的核位置。标记的类固醇的核结合是温度依赖的、类固醇特异的、可饱和的和细胞类型特异的。这些都是具有生物活性的雌激素受体的特征。在体外用10纳摩尔雌二醇处理6个细胞株中，有4个细胞株的孕酮特异性核结合增加，表明孕酮受体的功能被诱导。RNA印迹分析证明存在人类雌激素受体的信使RNA。这些数据表明，雌激素通过经典的雌激素受体介导的机制直接作用于人的骨细胞。
In seven strains of cultured normal human osteoblast-like cells, a mean of 1615 molecules of tritium-labeled 17.beta.-estradiol per cell nucleus could be bound to specific nuclear sites. The nuclear binding of the labeled steroid was temperature-dependent, steroid-specific, saturable, and cell type-specific. These are characteristics of biologically active estrogen receptors. Pretreatment with 10 nanomolar estradiol in vitro increased the specific nuclear binding of progesterone in four of six cell strains, indicating an induction of functional progesterone receptors. RNA blot analysis demonstrated the presence of messenger RNA for the human estrogen receptor. The data suggest that estrogen acts directly on human bone cells through a classical estrogen receptor-mediated mechanism.