Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization.
Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization.
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DOI：
10.1139/g98-055
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发表时间：
1998-08
期刊：
影响因子：
3.1
通讯作者：
Qin Chen;R. Conner;A. Laroche;J. Thomas
中科院分区：
文献类型：
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作者：
Qin Chen;R. Conner;A. Laroche;J. Thomas
基因组原位杂交(GISH)使用来自Thinopyrum长穗偃麦草(宿主)D.R.Dewey(基因组E，2n=14)、Thinopyrum bessarabicum(Savul.&Rayss)，A.Löve(基因组J，2n=14)和拟鹅观草属(M.Bieb.)利用A.Löve(基因组S，2n=14)，对中间偃麦草(Thinopyrum intermedia(宿主)Barkworth&D.R.Dewey(2n=6x=42))和南方偃麦草(Thinopyrum ponticum(Podp.)Barkworth&D.R.Dewey(2n=10x=70)。来自GISH的证据表明，六倍体Th中间含有J、Js和S基因组，其中J基因组与Th的E基因组有亲缘关系。LENGTUM和Th.Bessarabicum。S的基因组与S的基因组同源。Js基因组指的是改良的J型或E型染色体，其特征是在着丝粒附近存在S基因组特有的序列。十倍体Th.Ponticum只有两个基本基因组J和Js。Js基因组存在于Th.Interdium和Th.Ponticum与E或J基因组同源，但在着丝粒区域有较大差异，与S基因组探针有较强的杂交。根据GISH结果，给出了Th.中间层被重新命名为JJsS和Th。Ponticum改名为JJJJsJs。S、J和Js基因组之间亲缘关系的发现为利用S基因组DNA探针进行分子细胞遗传学分析监测Th有用性状的转移提供了有价值的标记。Interdium和Th.从洋葱到小麦。
Genomic in situ hybridization (GISH) using genomic DNA probes from Thinopyrum elongatum (Host) D.R. Dewey (genome E, 2n = 14), Thinopyrum bessarabicum (Savul. & Rayss) A. Löve (genome J, 2n = 14), and Pseudoroegneria strigosa (M. Bieb.) A. Löve (genome S, 2n = 14), was used to examine the genomic constitution of Thinopyrum intermedium (Host) Barkworth & D.R. Dewey (2n = 6x = 42) and Thinopyrum ponticum (Podp.) Barkworth & D.R. Dewey (2n = 10x = 70). Evidence from GISH indicated that hexaploid Th, intermedium contained the J, Js, and S genomes, in which the J genome was related to the E genome of Th. elongatum and the J genome of Th. bessarabicum. The S genome was homologous to the S genome of Ps. strigosa, while the Js genome referred to modified J- or E-type chromosomes distinguished by the presence of S genome specific sequences close to the centromere. Decaploid Th. ponticum had only the two basic genomes J and Js. The Js genome present in Th. intermedium and Th. ponticum was homologous with E or J genomes, but was quite distinct at centromeric regions, which can strongly hybridize with the S genome DNA probe. Based on GISH results, the genomic formula of Th. intermedium was redesignated JJsS and that of Th. ponticum was redesignated JJJJsJs. The finding of a close relationship among S, J, and Js genomes provides valuable markers for molecular cytogenetic analyses using S genome DNA probes to monitor the transfer of useful traits from Th. intermedium and Th. ponticum to wheat.