Molecular-level characterization of stratified extracellular polymeric substances of anammox sludge and its adsorption preference to refractory dissolved organic matter
Molecular-level characterization of stratified extracellular polymeric substances of anammox sludge and its adsorption preference to refractory dissolved organic matter
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DOI：
10.1016/j.energy.2020.118818
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发表时间：
2020-09
期刊：
影响因子：
9
通讯作者：
Li Zhang;Yongzhen Peng;S. Soda;Xiaowu Huang;Yifei Wang;Yanan Zhang
中科院分区：
文献类型：
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作者：
Li Zhang;Yongzhen Peng;S. Soda;Xiaowu Huang;Yifei Wang;Yanan Zhang
厌氧氨氧化是一种节能、环保的废水处理技术。污泥吸附是有机物进入厌氧氨氧化反应器后的一个重要过程。厌氧氨氧化污泥的胞外聚合物（EPS）对厌氧氨氧化活性起着关键作用。利用傅里叶变换离子回旋共振质谱（FT-ICR-MS）研究厌氧氨氧化污泥分层EPS的分子水平组成特征，探讨厌氧氨氧化处理过程中各层EPS对难降解溶解性有机物（DOM）的吸附偏好。结果表明，EPS吸附组分主要由脂类和蛋白质组成，其中CHO和CHON占80%。外层（泥，松散结合的EPS）优先吸附的化合物具有相对较高的芳香性和不饱和度，与含S的公式和木质素的主要成分。新产生的公式在出水被推断为与厌氧氨氧化处理性能。分子量较小、还原度较高的难降解DOM被EPS吸附，成为厌氧氨氧化污泥的一部分。
Anaerobic ammonium oxidation (anammox) is an energy saving and environmentally friendly technique for wastewater treatment. Sludge adsorption is an important process after organics enter the anammox reactor. The extracellular polymeric substances (EPS) of anammox sludge play a key role on the activity of anammox. This study utilized Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) to investigate molecular-level compositional characteristics of the stratified EPS of the anammox sludge, and to explore the adsorption preference of each EPS layer to refractory dissolved organic matter (DOM) during anammox treatment. Results showed that the adsorbed component by the tightly bound EPS layer was dominantly composed of lipids and proteins, with nearly 80% of formulas being CHO and CHON. The outer layers (slime, loosely bound-EPS) preferentially adsorbed the compounds with comparatively higher aromatic and unsaturated degrees, with S-containing formulas and lignin being the predominant components. The newly produced formulas in the effluent were inferred to be associated with the anammox treatment performance. The refractory DOM, with smaller molecular weight and high reductive degree, seemed to experience a single adsorption by the stratified EPS, and became part of the anammox sludge.