Cloning and developmental expression of the murine homolog of doublecortin
Cloning and developmental expression of the murine homolog of doublecortin
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DOI：
10.1006/bbrc.1998.9698
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发表时间：
1998-11-27
影响因子：
3.1
通讯作者：
Okubo, K
中科院分区：
文献类型：
--
作者：
Matsuo, N;Kawamoto, S;Okubo, K
在通过定量 3'-cDNA 收集分析新生小鼠海马中的活性基因时，我们鉴定了高度保守的双皮质蛋白同源物，它是 X 连锁无脑畸形 (XLIS) 和皮质下层状异位 (SCLH) 综合征的致病基因。 m-双皮质素 cDNA 包含与 hs-双皮质素同源的近 8 kb 3' UTR，并且被定位到 X 染色体。 m-双皮质素的表达仅限于发育中的中枢神经系统，尤其是皮质板，支持 XLIS/SCLH 综合征与大脑皮层神经元迁移停滞相关。 m-双皮质素 mRNA 在神经元前体增殖的心室区不存在，有趣的是，它在这种综合征患者通常不受影响的各种大脑结构中被发现。 (C) 1998 年学术出版社。
While analyzing active genes in neonatal mouse hippocampus by quantitative 3'-cDNA collection, we identified a highly conserved murine homolog of doublecortin, the causative gene of X-linked lissencephaly (XLIS) and subcortical laminar heterotopia (SCLH) syndrome. The m-doublecortin cDNA contains nearly 8 kb 3' UTR homologous to hs-doublecortin and it was mapped to the X chromosome. The expression of m-doublecortin is limited to the developing CNS, especially the cortical plate, supporting that XLIS/SCLH syndrome is associated with an arrest of neuronal migration in the cerebral cortex. The m-doublecortin mRNA was absent in the ventricular zone where neuronal precursors proliferate, and interestingly it was found in various brain structures that are not typically affected in patients with this syndrome. (C) 1998 Academic Press.