PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2.
PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2.
复制标题
PhoR/PhoP双组分调控系统影响枯草芽孢杆菌NCD-2的生防能力。
DOI：
10.1590/s1415-47572010005000032
复制
发表时间：
2010-04
影响因子：
2.1
通讯作者：
Ma P
中科院分区：
文献类型：
--
作者：
Guo Q;Li S;Lu X;Li B;Ma P
枯草芽孢杆菌NCD-2菌株是防治棉花黄萎病和棉花疮痂病的重要生防菌。菌株NCD-2的突变体，命名为M216，其对大丽轮枝菌和R. solani，通过mini-Tn 10诱变和离体毒力筛选筛选。在突变体中插入的基因被克隆和鉴定为phoR基因，其编码PhoP/PhoR双组分系统中的传感器激酶。与野生型菌株相比，突变株在低磷培养基中的AP酶活性显著降低，而在高磷培养基中的AP酶活性无明显差异。该突变体在有机磷酸盐琼脂上生长也更慢，并失去了其磷脂酰胆碱溶解能力。与野生型菌株相比，突变体菌株对棉花立枯病的抑制作用和在棉花根际的定殖作用也有所降低。所有这些特性都可以通过M216突变体中phoR基因的互补而部分恢复。
The Bacillus subtilis strain NCD-2 is an important biocontrol agent against cotton verticillium wilt and cotton sore shin in the field, which are caused by Verticillium dahliae Kleb and Rhizoctonia solani Kuhn, respectively. A mutant of strain NCD-2, designated M216, with decreased antagonism to V. dahliae and R. solani, was selected by mini-Tn10 mutagenesis and in vitro virulence screening. The inserted gene in the mutant was cloned and identified as the phoR gene, which encodes a sensor kinase in the PhoP/PhoR two-component system. Compared to the wild-type strain, the APase activities of the mutant was decreased significantly when cultured in low phosphate medium, but no obvious difference was observed when cultured in high phosphate medium. The mutant also grew more slowly on organic phosphate agar and lost its phosphatidylcholine-solubilizing ability. The suppression of cotton seedling damping-off in vivo and colonization of the rhizosphere of cotton also decreased in the mutant strain when compared with the wild type strain. All of these characteristics could be partially restored by complementation of the phoR gene in the M216 mutant.