EARLY EVENTS IN EPSTEIN-BARR-VIRUS INFECTION OF HUMAN LYMPHOCYTES-B
EARLY EVENTS IN EPSTEIN-BARR-VIRUS INFECTION OF HUMAN LYMPHOCYTES-B
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DOI：
10.1016/0042-6822(91)90893-g
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发表时间：
1991-04-01
期刊：
影响因子：
3.7
通讯作者：
KIEFF, E
中科院分区：
文献类型：
--
作者：
ALFIERI, C;BIRKENBACH, M;KIEFF, E
分析急性感染后3d内EB病毒（EBV）和B淋巴细胞变化的时序。到16小时，平均感染的淋巴细胞具有1个EBV附加体。核蛋白-2（EBNA-2）和EBNA-前导蛋白（-LP）在12小时内被检测到，并在32小时内在淋巴母细胞系（LCL）中处于稳定的EBV感染水平。在12小时时，所有EBNA-LP和EBNA-2 RNA均从Pw启动子起始。到36小时，显著的EBNA-LP和EBNA-2 RNA部分从上游Pc启动子起始。在整个急性感染过程中，相似比例的潜在双顺反子EBNA-LP mRNA具有第一外显子剪接，这将导致EBNA-LP翻译。到36小时，c-myc RNA被瞬时诱导，CD 21和CD 23 RNA开始增加。这与低水平EBNA-1、EBNA-3A、B和C以及潜伏膜蛋白-1（LMP-1）表达相一致。到46小时，EBNA-1、EBNA-3和LMP-1接近通常在LCL中发现的水平，并且大部分淋巴细胞处于S期。这些结果与EBNA-2（或EBNA-LP）在调节病毒和细胞基因表达中的关键作用相一致。EBV编码的小RNA（EBER）的高水平表达延迟超过36小时，因此可能被其他病毒或细胞基因激活。一个65 kDa的病毒体蛋白持续在急性感染的细胞。该蛋白可能是病毒或细胞基因表达的介导物。
The sequence of Epstein-Barr virus (EBV) and B lymphocyte changes in the 3 days following acute infection was analyzed. By 16 hr the average infected lymphocyte had 1 EBV episome. Nuclear protein-2 (EBNA-2) and EBNA-leader protein (-LP) were detected by 12 hr, and by 32 hr were at the levels of stable EBV infection in lymphoblastoid cell lines (LCLs). At 12 hr, all EBNA-LP and EBNA-2 RNAs were initiated from the Pw promoter. By 36 hr a significant EBNA-LP and EBNA-2 RNA fraction initiated from the upstream Pc promoter. Throughout acute infection, a similar fraction of potentially bicistronic EBNA-LP mRNAs had first exon splices which would result in EBNA-LP translation. By 36 hr c-myc RNA was transiently induced, and CD21 and CD23 RNAs were beginning to increase. This coincided with low-level EBNA-1, EBNA-3A, B, and C, and latent membrane protein-1 (LMP-1) expression. By 46 hr, EBNA-1, the EBNA-3s, and LMP-1 were near the levels ordinarily found in LCLs and a substantial fraction of lymphocytes were in S phase. These results are compatible with a key role for EBNA-2 (or EBNA-LP) in regulating virus and cell gene expression. High-level expression of the EBV-encoded small RNAs, EBERs, was delayed beyond 36 hr and may, therefore, be activated by other virus or cell genes. A 65-kDa virion protein persisted in acutely infected cells. This protein could be a mediator of virus or cell gene expression.