The control of cyclin B1 mRNA translation during mouse oocyte maturation
The control of cyclin B1 mRNA translation during mouse oocyte maturation
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DOI：
10.1006/dbio.2000.9669
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发表时间：
2000-05-01
影响因子：
2.7
通讯作者：
Richter, JD
中科院分区：
文献类型：
--
作者：
Tay, J;Hodgman, R;Richter, JD
在成熟的小鼠卵母细胞中，生发囊泡破裂（GVBD）后的减数分裂成熟需要蛋白质合成。虽然发生这一进展必须合成的不同蛋白质的数量尚不清楚，但其中至少有一种似乎是细胞周期蛋白 B1，即 M 期促进因子的调节亚基。在这里，我们研究了小鼠卵母细胞成熟过程中细胞周期蛋白 B1 mRNA 翻译控制的机制。我们发现富含 U 的细胞质多腺苷酸化元件 (CPE)（细胞周期蛋白 B1 mRNA 3' UTR 中的顺式元件）介导 GV 期卵母细胞的翻译抑制。 CPE 对于细胞质多腺苷酸化也是必需的，细胞质多腺苷酸化会在卵母细胞成熟过程中刺激翻译。向卵母细胞注射细胞周期蛋白 B1 反义 RNA，可能会阻止因子与 CPE 的结合，从而延迟细胞质多聚腺苷酸化以及从 GVBD 到中期 II 的转变。 CPEB 与细胞周期蛋白 B1 CPE 相互作用，存在于整个减数分裂成熟过程中，在中期 I 磷酸化。这些数据表明 CPEB 参与细胞周期蛋白 B1 mRNA 的抑制和刺激，并表明该蛋白的磷酸化可能参与调节其活性。 (C) 2000 年学术出版社。
In maturing mouse oocytes, protein synthesis is required for meiotic maturation subsequent to germinal vesicle breakdown (GVBD). While the number of different proteins that must be synthesized for this progression to occur is unknown, at least one of them appears to be cyclin B1, the regulatory subunit of M-phase-promoting factor. Here, we investigate the mechanism of cyclin B1 mRNA translational control during mouse oocyte maturation. We show that the U-rich cytoplasmic polyadenylation element (CPE), a cis element in the 3' UTR of cyclin B1 mRNA, mediates translational repression in GV-stage oocytes. The CPE is also necessary for cytoplasmic polyadenylation, which stimulates translation during oocyte maturation. The injection of oocytes with a cyclin B1 antisense RNA, which probably precludes the binding of a factor to the CPE, delays cytoplasmic polyadenylation as well as the transition from GVBD to metaphase II. CPEB, which interacts with the cyclin B1 CPE and is present throughout meiotic maturation, becomes phosphorylated at metaphase I. These data indicate that CPEB is involved in both the repression and the stimulation of cyclin B1 mRNA and suggest that the phosphorylation of this protein could be involved in regulating its activity. (C) 2000 Academic Press.