Stem/progenitor cells from inflamed human dental pulp retain tissue regeneration potential.
Stem/progenitor cells from inflamed human dental pulp retain tissue regeneration potential.
复制标题
DOI：
10.2217/rme.10.30
复制
发表时间：
2010-07
影响因子：
2.7
通讯作者：
Huang GT
中科院分区：
文献类型：
--
作者：
Alongi DJ;Yamaza T;Song Y;Fouad AF;Romberg EE;Shi S;Tuan RS;Huang GT
有效的干/祖细胞已从正常人牙髓中分离出来，称为牙髓干细胞（DPSC）。然而，目前尚不清楚这些细胞是否存在于发炎的牙髓（IP）中。确定是否可以从IP中鉴定和分离DPSC;如果可以成功培养，它们是否保留体内组织再生潜力。从新鲜收集的正常牙髓（NP）和IP的DPSCs进行了体外表征，并使用体内研究模型测试其组织再生潜力。免疫组化结果显示，与NP相比，IP表达更高水平的间充质干细胞标志物STRO-1、CD 90、CD 105和CD 146（p < 0.05）。流式细胞术分析显示，来自NP和IP的DPSC表达中等至高水平的CD 146、阶段特异性胚胎抗原-4、CD 73和CD 166。DPSCs-IP的总群体倍增（44.6 ± 2.9）低于DPSCs-NP的总群体倍增（58.9 ± 2.5）（p < 0.05），并且基于培养物中的矿物质沉积，DPSCs-IP与DPSCs-NP相比似乎具有降低的成骨/牙本质潜力。尽管如此，DPSCs-IP形成的牙髓/牙本质复合物类似于DPSCs-NP移植到免疫功能低下的小鼠。DPSCs-IP可以被分离，并且它们的间充质干细胞标志物谱与来自NP的标志物谱相似。虽然DPSCs-IP的一些干细胞特性被改变，但来自一些样品的细胞在体内组织再生中仍然是有效的。
Potent stem/progenitor cells have been isolated from normal human dental pulps termed dental pulp stem cells (DPSCs). However, it is unknown whether these cells exist in inflamed pulps (IPs). To determine whether DPSCs can be identified and isolated from IPs; and if they can be successfully cultured, whether they retain tissue regeneration potential in vivo. DPSCs from freshly collected normal pulps (NPs) and IPs were characterized in vitro and their tissue regeneration potential tested using an in vivo study model. The immunohistochemical analysis showed that IPs expressed higher levels of mesenchymal stem cell markers STRO-1, CD90, CD105 and CD146 compared with NPs (p < 0.05). Flow cytometry analysis showed that DPSCs from both NPs and IPs expressed moderate to high levels of CD146, stage-specific embryonic antigen-4, CD73 and CD166. Total population doubling of DPSCs-IPs (44.6 ± 2.9) was lower than that of DPSCs-NPs (58.9 ± 2.5) (p < 0.05), and DPSCs-IPs appeared to have a decreased osteo/dentinogenic potential compared with DPSCs-NPs based on the mineral deposition in cultures. Nonetheless, DPSCs-IPs formed pulp/dentin complexes similar to DPSCs-NPs when transplanted into immunocompromised mice. DPSCs-IPs can be isolated and their mesenchymal stem cell marker profiles are similar to those from NPs. Although some stem cell properties of DPSCs-IPs were altered, cells from some samples remained potent in tissue regeneration in vivo.