Small differences in electrophoretic mobility among circularly permuted sequence isomers of duck hepatitis B virus linear, single-stranded DNA.
Small differences in electrophoretic mobility among circularly permuted sequence isomers of duck hepatitis B virus linear, single-stranded DNA.
复制标题
鸭乙型肝炎病毒线性单链 DNA 的循环排列序列异构体之间的电泳迁移率存在微小差异。
DOI：
10.1111/j.1574-6968.1995.tb07574.x
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发表时间：
1995
影响因子：
2.1
通讯作者：
Newbold,JE
中科院分区：
文献类型：
--
作者：
Tencza,MG;Newbold,JE
Two linear minus-strand viral DNAs, disparate in size by 10 nucleotides, were isolated from duck hepatitis B virus infected tissues and observed to migrate differently in nondenaturing agarose gels. We examined this phenomenon using both synthetic and cloned viral DNAs and discovered that distinct, circularly permuted, linear isomers of single-stranded DNA could have slightly different electrophoretic mobilities under nondenaturing conditions. This finding reveals a novel feature for consideration in assessing the conformations of native or renatured single-stranded nucleic acids. The study also suggests that the virion-derived minus-strand DNAs of theavihepadnaviridaemay necessarily possess a minimal secondary structure.
