STRUCTURE OF THE LINKAGE UNITS BETWEEN RIBITOL TEICHOIC-ACIDS AND PEPTIDOGLYCAN
STRUCTURE OF THE LINKAGE UNITS BETWEEN RIBITOL TEICHOIC-ACIDS AND PEPTIDOGLYCAN
复制标题
DOI：
10.1128/jb.161.1.299-306.1985
复制
发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
ITO, E
中科院分区：
文献类型：
--
作者：
KOJIMA, N;ARAKI, Y;ITO, E
本文研究了金黄色葡萄球菌H和209 P、枯草芽孢杆菌W23和AHU 1390细胞壁中核糖醇磷壁酸与肽聚糖连接区的结构。磷壁酸连接的糖制剂通过在pH 2.5下加热从细胞壁获得，含有少量的甘露糖胺和甘油。在温和的碱处理下，每种磷壁酸连接的糖制备物被分解为鉴定为N-乙酰甘露糖胺β（1- 4）N-乙酰葡糖胺的二糖和含有甘油残基的核糖醇磷壁酸部分。采用Smith降解法研究了S. aureus和B.枯草芽孢杆菌（Bacillus subtilis）分离得到的片段分别为1，2-亚乙基二醇磷酸-（甘油磷酸）3-N-乙酰甘露糖胺β（1----4）N-乙酰木糖醇和1，2-亚乙基二醇磷酸-（甘油磷酸）2-N-乙酰甘露糖胺β（1----4）N-乙酰木糖醇。通过分析磷壁酸-糖肽复合物的NaIO 4氧化得到的连接单元结合的糖肽，证实了二糖单元与肽聚糖的结合。温和的碱处理的连接单元结合的糖肽产生的二糖连接的糖肽，这给出了二糖和磷酸化的糖肽在温和的酸处理。因此，可以认为，S. aureus和B.枯草芽孢杆菌分别通过连接单元（甘油磷酸）3-N-乙酰甘露糖胺基β（1-4）N-乙酰葡糖胺和（甘油磷酸）2-N-乙酰甘露糖胺基β（1-4）N-乙酰葡糖胺与肽聚糖连接。
The structure of the linkage regions between ribitol teichoic acids and peptidoglycan in the cell walls of Staphylococcus aureus H and 209P and Bacillus subtilis W23 and AHU 1390 was studied. Teichoic acid-linked saccharide preparations obtained from the cell walls by heating at pH 2.5 contained mannosamine and glycerol in small amounts. On mild alkali treatment, each teichoic acid-linked saccharide preparation was split into a disaccharide identified as N-acetylmannosaminyl beta(1----4)N-acetylglucosamine and the ribitol teichoic acid moiety that contained glycerol residues. The Smith degradation of reduced samples of the teichoic acid-linked saccharide preparations from S. aureus and B. subtilis gave fragments characterized as 1,2-ethylenediol phosphate-(glycerolphosphate)3-N-acetylmannosaminyl beta(1----4)N- -acetylxylosaminitol and 1,2-ethylenediolphosphate-(glycerol phosphate)2-N-acetylmannosaminyl beta(1----4)N-acetylxylosaminitol, respectively. The binding of the disaccharide unit to peptidoglycan was confirmed by the analysis of linkage-unit-bound glycopeptides obtained from NaIO4 oxidation of teichoic acid-glycopeptide complexes. Mild alkali treatment of the linkage-unit-bound glycopeptides yielded disaccharide-linked glycopeptides, which gave the disaccharide and phosphorylated glycopeptides on mild acid treatment. Thus, it is concluded that the ribitol teichoic acid chains in the cell walls of the strains of S. aureus and B. subtilis are linked to peptidoglycan through linkage units, (glycerol phosphate)3-N-acetylmannosaminyl beta(1----4)N-acetylglucosamine and (glycerol phosphate)2-N-acetylmannosaminyl beta(1----4)N-acetylglucosamine, respectively.