Nucleotide sequence and transcription of a bacteriophage 29 early promoter.
Nucleotide sequence and transcription of a bacteriophage 29 early promoter.
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噬菌体 29 早期启动子的核苷酸序列和转录。
DOI：
10.1016/s0021-9258(18)88921-9
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
G. Spiegelman
中科院分区：
文献类型：
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作者：
K. F. Dobinson;G. Spiegelman
我们研究了枯草杆菌RNA聚合酶启动子在噬菌体phi 29 DNA上的体外和体内转录。该启动子被鉴定为早期启动子，因为它在体外由未感染的B转录。含有枯草杆菌sigma 55的RNA聚合酶;在感染后7分钟和在氯霉素存在下体内转录;并且在标准phi 29图谱上从右向左转录。报告了启动子的核苷酸序列和RNA合成的起始位点。我们还研究了使用单轮径流转录测定的RNA合成起始的动力学。发现引发的总速率为1.6 × 10（6）M-1 s-1，而不稳定聚合酶-促进剂复合物转化为稳定聚合酶-促进剂复合物的速率为0.049 s-1。这些值与大肠杆菌RNA聚合酶的类似启动子的值相当。
We have studied the in vitro and in vivo transcription of a promoter for Bacillus subtilis RNA polymerase on bacteriophage phi 29 DNA. The promoter is identified as an early promoter as it is transcribed in vitro by uninfected B. subtilis sigma 55-containing RNA polymerase; is transcribed in vivo at both 7 min after infection and in the presence of chloramphenicol; and is transcribed right to left on the standard phi 29 map. The nucleotide sequence of the promoter and the initiation site for RNA synthesis are reported. We have also examined the kinetics of RNA synthesis initiation using a single round run-off transcription assay. The overall rate of initiation was found to be 1.6 X 10(6) M-1 s-1 while the rate of conversion of unstable to stable polymerase-promoter complexes was 0.049 s-1. These values are comparable to those for similar promoters for Escherichia coli RNA polymerase.