Murine gammaherpesvirus-68 ORF28 encodes a non-essential virion glycoprotein
Murine gammaherpesvirus-68 ORF28 encodes a non-essential virion glycoprotein
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DOI：
10.1099/vir.0.80661-0
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发表时间：
2005-04-01
影响因子：
3.8
通讯作者：
Stevenson, PG
中科院分区：
文献类型：
--
作者：
May, JS;Coleman, HM;Stevenson, PG
鼠γ疱疹病毒-68（MHV-68）ORF 28是一个功能未知的γ疱疹病毒特异性基因。对表位标记的ORF 28蛋白的分析表明，它是膜结合的，并以N-糖基化、O-糖基化和非糖基化的形式掺入病毒粒子中。小ORF 28细胞外结构域的广泛糖基化-大多数形式的蛋白质似乎主要是碳水化合物重量-表明ORF 28的主要功能是将各种聚糖附着到病毒体表面。缺乏ORF 28的MHV-68在体外和体内显示正常的裂解复制和正常的潜伏期建立。因此，MHV-68 ORF 28编码一种小的、膜结合且广泛糖基化的病毒粒子蛋白，其功能对于正常的单循环宿主定植完全是不可或缺的。
Murine gammaherpesvirus-68 (MHV-68) ORF28 is a gammaherpesvirus-specific gene of unknown function. Analysis of epitope-tagged ORF28 protein indicated that it was membrane-associated and incorporated into virions in N-glycosylated, O-glycosylated and unglycosylated forms. The extensive glycosylation of the small ORF28 extracellular domain - most forms of the protein appeared to be mainly carbohydrate by weight - suggested that a major function of ORF28 is to attach a variety of glycans to the virion surface. MHV-68 lacking ORF28 showed normal lytic replication in vitro and in vivo and normal latency establishment. MHV-68 ORF28 therefore encodes a small, membrane-bound and extensively glycosylated virion protein, whose function is entirely dispensable for normal, single-cycle host colonization.