Catecholaminergic neurons in rat dorsal motor nucleus of vagus project selectively to gastric corpus
Catecholaminergic neurons in rat dorsal motor nucleus of vagus project selectively to gastric corpus
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DOI：
10.1152/ajpgi.2001.280.3.g361
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发表时间：
2001-03-01
影响因子：
4.5
通讯作者：
Travagli, RA
中科院分区：
文献类型：
--
作者：
Guo, JFJ;Browning, KN;Travagli, RA
大鼠迷走神经背侧运动核（DMV）内的一氧化氮合酶免疫反应（NOS-IR）神经元选择性地投射到胃底，可能参与控制胃扩张的迷走神经反射。本研究旨在确定酪氨酸羟化酶免疫反应（TH-IR）DMV神经元的胃投射，这些神经元是否共定位NOS-IR，以及它们是否在食管扩张后被激活。在胃底、胃体或胃窦/幽门的肌壁内注射逆行示踪剂后，在去除脑干并进行TH-和NOS-IR处理之前，识别出胃投射神经元。胃体投射神经元的TH- IR比例显著高于胃底和胃窦/幽门投射神经元（分别为14%比4%和2%，P< 0.05）。NOS和TH-IR在胃投射神经元中未见共定位。在间歇性食管球囊扩张后测试c-Fos激活的大鼠中，在DMV神经元中未观察到与TH-IR的共定位。这些结果表明，DMV尾侧的TH- IR神经元主要投射到胃体，构成一个不同于氮能迷走神经元的亚群，并且在食管扩张时不被激活。
Nitric oxide synthase-immunoreactive (NOS-IR) neurons in the rat caudal dorsal motor nucleus of the vagus (DMV) project selectively to the gastric fundus and may be involved in vagal reflexes controlling gastric distension. This study aimed to identify the gastric projections of tyrosine hydroxylase-immunoreactive (TH-IR) DMV neurons, whether such neurons colocalize NOS-IR, and if they are activated after esophageal distension. Gastric-projecting neurons were identified after injection of retrograde tracers into the muscle wall of the gastric fundus, corpus, or antrum/pylorus before removal and processing of the brain stems for TH- and NOS-IR. A significantly higher proportion of corpus-compared with fundus- and antrum/pylorus- projecting neurons were TH- IR (14% compared with 4% and 2%, respectively, P< 0.05). Colocalization of NOS- and TH- IR was never observed in gastric-projecting neurons. In rats tested for c-Fos activation after intermittent esophageal balloon distension, no colocalization with TH- IR was observed in DMV neurons. These findings suggest that TH- IR neurons in the caudal DMV project mainly to the gastric corpus, constitute a subpopulation distinct from that of nitrergic vagal neurons, and are not activated on esophageal distension.