Expression of the cholecystokinin gene in rat brain during development.
Expression of the cholecystokinin gene in rat brain during development.
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发育过程中大鼠大脑中胆囊收缩素基因的表达。
DOI：
10.1159/000111609
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发表时间：
1987
影响因子：
2.9
通讯作者：
Wyatt,RJ
中科院分区：
文献类型：
--
作者：
Duchemin,AM;Quach,TT;Iadarola,MJ;Deschenes,RJ;Schwartz,JP;Wyatt,RJ
采用procck特异性cDNA探针定量研究了大鼠脑中胆囊收缩素（CCK）编码基因的表达。此外，采用c端特异性放射免疫分析法测定cck免疫反应肽。通过琼脂糖凝胶电泳分析大鼠脑中Poly(A)+富集的RNA是否存在CCK mRNA，然后将其转移到硝化纤维素上，杂交到32p标记的CCK cDNA探针上。观察到大约700个核苷酸的RNA，不同发育阶段的样品之间没有明显的CCK mRNA差异。大脑preproCCK mRNA在胚胎第14天检测到，并持续增加，直到出生后第14天达到最大浓度。在年轻的成年大鼠中，水平略有下降。cck免疫反应肽从胚胎第21天开始在样本中被检测到，但只有在出生后才出现明显的发育，在出生后的前2周出现rajjid增加。该肽在年轻成年大鼠中含量最高。
The expression of the gene encoding for cholecystokinin (CCK) was studied pre- and postnatally in rat brain with a preproCCK-specific cDNA probe to quantitate mRNA. In addition, a C-terminal-specific radioimmunoassay was used to measure CCK-immunoreactive peptides. Poly(A)+-enriched RNA from rat brain was analyzed for the presence of CCK mRNA by agarose gel electrophoresis, followed by transfer to nitrocellulose and hybridization to a32P-labeled CCK cDNA probe. An RNA of approximately 700 nucleotides was observed and there were no obvious CCK mRNA differences between samples taken at the different developmental stages. Brain preproCCK mRNA was detectable by embryonic day 14 and increased continuously until postnatal day 14 when it reached maximal concentrations. In young adult rats, levels were slightly decreased. CCK-immunoreactive peptides began to be detected in the samples from embryonic day 21, but marked development was seen only postnatally with a rajjid increase occurring during the first 2 weeks after birth. The highest content of the peptide was present in young adult rats.