Functional analysis of the 37 kDa inner envelope membrane polypeptide in chloroplast biogenesis using a Ds-tagged Arabidopsis pale-green mutant
Functional analysis of the 37 kDa inner envelope membrane polypeptide in chloroplast biogenesis using a Ds-tagged Arabidopsis pale-green mutant
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DOI：
10.1046/j.1365-313x.2003.01763.x
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发表时间：
2003-06-01
期刊：
影响因子：
7.2
通讯作者：
Shinozaki, K
中科院分区：
文献类型：
--
作者：
Motohashi, R;Ito, T;Shinozaki, K
为了研究叶绿体发育相关核基因的功能，我们利用基于玉米Ac/Ds元件的双组分转座子系统作为诱变剂，对拟南芥的白化病和淡绿突变体进行了系统分析。其中一个淡绿色突变体，白化病或淡绿色绿色突变体1（命名为apg 1），在土壤中发芽时，不能存活超过幼苗阶段。apg 1植物的叶绿体中含有数量减少的lamphine，叶绿素水平降低。通过在apg 1中插入Ds转座子来破坏编码叶绿体内被膜的37 kDa多肽前体的基因。37 kDa的蛋白质具有部分序列相似性的S -腺苷甲硫氨酸依赖性甲基转移酶。apg 1植物缺乏质体醌（PQ），表明APG 1蛋白参与PQ生物合成的甲基化步骤，这是定位在包膜膜。我们的研究结果表明，在拟南芥叶绿体发育的叶绿体内被膜的37 kDa蛋白的重要性。
To study the functions of the nuclear genes involved in chloroplast development, we systematically analyzed albino and pale-green Arabidopsis thaliana mutants by using a two-component transposon system based on the Ac/Ds element of maize as a mutagen. One of the pale-green mutants, albino or pale green mutant 1 (designated as apg1), did not survive beyond the seedling stage, when germinated on soil. The chloroplasts of the apg1 plants contained decreased numbers of lamellae with reduced levels of chlorophyll. A gene encoding a 37 kDa polypeptide precursor of the chloroplast inner envelope membrane was disrupted by insertion of the Ds transposon in apg1. The 37 kDa protein had partial sequence similarity to the S -adenosylmethionine-dependent methyltransferase. The apg1 plants lacked plastoquinone (PQ), suggesting that the APG1 protein is involved in the methylation step of PQ biosynthesis, which is localized at the envelope membrane. Our results demonstrate the importance of the 37 kDa protein of the chloroplast inner envelope membrane for chloroplast development in Arabidopsis.