ANTIBODY PREVALENCE AND MOLECULAR IDENTIFICATION OF BABESIA SPP. IN ROE DEER IN FRANCE
ANTIBODY PREVALENCE AND MOLECULAR IDENTIFICATION OF BABESIA SPP. IN ROE DEER IN FRANCE
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DOI：
10.7589/0090-3558-48.2.416
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发表时间：
2012-04-01
影响因子：
1.3
通讯作者：
Chauvin, Alain
中科院分区：
文献类型：
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作者：
Bastian, Suzanne;Jouglin, Maggy;Chauvin, Alain
在2004年对在不同景观中自由放牧的马鹿进行的一项区域范围的血清学研究中，我们发现58%的动物(406只动物中的237只)对巴贝斯虫分歧抗原抗体阳性。对2004-08年两个围栏林区活体捕获的327头马鹿的血清学和感染状况进行了分析。在此期间，连续两年，在这些封闭种群中，92%和94%的鹿对分枝杆菌抗体阳性。巴贝斯虫在131只被困动物(40%)的自体红细胞培养中被分离出来。采用聚合酶链式反应(PCR)限制性片段长度多态性方法对76株沙门氏菌进行了18S核糖体亚基基因(18株)和bd37基因(60株)的分子分型。结果表明，两种森林中都存在卡氏双歧杆菌和静脉双歧杆菌的持续共循环，可能存在两种动物的混合感染。未检测到分枝杆菌感染。对分离到的15株细菌进行18S rRNA基因部分序列测定，确定为卡氏假单胞菌。通过对bd37基因的聚合酶链式反应检测，本研究中的9个脉杆菌分离株均为阴性，而15个确认分离株和50个推定的Capreoli分离株表现出非常不同的限制性内切酶图谱，与已知的分枝杆菌中bd37的限制性内切酶图谱不同。两个分离株显示出相互矛盾的结果，提示存在混合感染。
In a region-wide serologic study carried out in 2004 on free-ranging hunted roe deer in various landscapes, we found that 58% of the animals (237 out of 406) were antibody positive for Babesia divergens antigen. Serologic and infection status was also analyzed for 327 roe deer live-trapped in two fenced forest areas over 5 yr (2004-08). For two consecutive years during this period, 92 and 94% of the deer in these closed populations were antibody-positive for B. divergens. Babesia spp. were isolated in autologous red blood cell culture for 131 of the trapped animals (40%). Molecular typing was done on 76 isolates with polymerase chain reaction (PCR) restriction fragment length polymorphism methods targeted at the 18S ribosomal subunit gene (18 isolates) and the Bd37 gene coding for a merozoite surface antigen implicated in a protective response (60 isolates). Results indicated continuous cocirculation of B. capreoli and B. venatorum in both forests and possible coinfection of animals with both species. No infection with B. divergens was detected. Fifteen isolates were confirmed to be B. capreoli by sequencing part of the 18S rRNA gene. Using PCR detection of the Bd37 gene, all nine isolates of B. venatorum in this study were negative, whereas the 15 confirmed and 50 putative B. capreoli isolates showed very variable restriction profiles, distinct from those known for Bd37 in B. divergens. Two isolates showed conflicting results, suggestive of mixed infection.