MUTANT GENE THAT CHANGES PROTEIN COMPOSITION + INCREASES LYSINE CONTENT OF MAIZE ENDOSPERM
MUTANT GENE THAT CHANGES PROTEIN COMPOSITION + INCREASES LYSINE CONTENT OF MAIZE ENDOSPERM
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DOI：
10.1126/science.145.3629.279
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发表时间：
1964-01-01
期刊：
影响因子：
56.9
通讯作者：
BATES, LS
中科院分区：
文献类型：
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作者：
MERTZ, ET;NELSON, OE;BATES, LS
前期试验表明，OPAQUE-2突变基因纯合的玉米种子胚乳中的赖氨酸含量高于正常籽粒。作为关键试验，将回交后代分为不透明2号籽粒和正常籽粒，分离胚乳，测定氨基酸含量。与正常种子相比，不透明-2胚乳具有不同的氨基酸模式和69%的赖氨酸含量。这些变化的主要原因是突变胚乳的酸溶部分合成了碱性氨基酸含量较高的蛋白质。伴随而来的是玉米醇溶蛋白与谷蛋白的比例降低。
Pre-liminary tests have shown that the endosperms of maize seeds homo-zygous for the opaque-2 mutant gene have a higher lysine content than normal kernels. As a critical test, a backcross progeny was divided into opaque-2 and normal kernels, the endosperms separated, and the amino acids determined. The opaque-2 endosperms had a different amino acid pattern and 69% more lysine than the normal seeds. The major reason for these changes is the synthesis of proteins with a greater content of basic amino acids in the acid-soluble fraction of the mutant endosperm. This is accompanied by a reduction in the ratio of zein to glutelin.