Anti-inflammatory effects of curcumin in experimental spinal cord injury in rats
Anti-inflammatory effects of curcumin in experimental spinal cord injury in rats
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DOI：
10.1007/s00011-014-0710-z
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发表时间：
2014-05-01
影响因子：
6.7
通讯作者：
Liang, Weibang
中科院分区：
文献类型：
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作者：
Jin, Wei;Wang, Jing;Liang, Weibang
抗氧化转录因子核因子红细胞2相关因子2（Nrf 2）在脊髓损伤（SCI）诱导的炎症反应中起重要作用。本研究的目的是测试姜黄素，一种新的Nrf 2激活剂，是否可以保护脊髓免受SCI诱导的炎症damage.Adult雄性Sprague-Dawley大鼠在T8-T9进行椎板切除术和压缩与血管夹。收集跨越损伤部位约0.8cm的脊髓用于测试。分为三组：（a）假手术组;（B）SCI组;和（c）SCI +姜黄素组。我们检测了Nrf 2和核因子κ B，（NF-κ B）结合活性（电泳迁移率变动测定法），肿瘤坏死因子-α、白介素-1 β和白介素-6浓度（酶联免疫吸附测定法），后肢运动功能（Basso、Beattie和Bresnahan评级法），脊髓水肿（湿/干重法），姜黄素诱导Nrf 2活性可明显降低损伤脊髓NF-κ B B的活化和炎性细胞因子的产生。姜黄素的管理也显着改善了继发性脊髓损伤，如所示的运动功能障碍，脊髓水肿，和apoptosis.Post-SCI姜黄素管理减弱在受伤的脊髓炎症反应，这可能是一个机制，姜黄素改善SCI后的结果。
Antioxidant transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) has been shown in our previous studies to play an important role in protection against spinal cord injury (SCI) induced inflammatory response. The objective of this study was to test whether curcumin, a novel Nrf2 activator, can protect the spinal cord against SCI-induced inflammatory damage.Adult male Sprague-Dawley rats were subjected to laminectomy at T8-T9 and compression with a vascular clip. The spinal cords spanning the injury site about 0.8 cm were collected for testing. There were three groups: (a) sham group; (b) SCI group; and (c) SCI + curcumin group. We measured Nrf2 and nuclear factor kappa B (NF-kappa B) binding activities by electrophoretic mobility shift assay, concentrations of tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 by enzyme-linked immunosorbent assay, hindlimb locomotion function by Basso, Beattie, and Bresnahan rating, spinal cord edema by the wet/dry weight method, and apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling analysis.Induction of the Nrf2 activity by curcumin markedly decreased NF-kappa B activation and inflammatory cytokines production in the injured spinal cord. Administration of curcumin also significantly ameliorated the secondary spinal cord damage, as shown by decreased severity of locomotion deficit, spinal cord edema, and apoptosis.Post-SCI curcumin administration attenuates the inflammatory response in the injured spinal cord, and this may be a mechanism whereby curcumin improves the outcome following SCI.