Nerve growth factor-immobilized electrically conducting fibrous scaffolds for potential use in neural engineering applications.
Nerve growth factor-immobilized electrically conducting fibrous scaffolds for potential use in neural engineering applications.
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DOI：
10.1109/tnb.2011.2159621
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发表时间：
2012-03
影响因子：
3.9
通讯作者：
Schmidt CE
中科院分区：
文献类型：
--
作者：
Lee JY;Bashur CA;Milroy CA;Forciniti L;Goldstein AS;Schmidt CE
同时显示多种线索的工程支架在神经组织再生中是非常理想的。为此，我们开发了一种神经组织工程支架，它具有亚微米级的特征、电导率和神经营养活性。具体来说，电纺丝聚乳酸-共羟基乙酸（PLGA）纳米纤维被一层纳米厚的具有羧基的导电聚吡咯（PPy）涂层所覆盖。然后，将神经生长因子（NGF）化学固定在纤维表面。这些NGF固定的py -coated PLGA （NGF- ppyplga）纤维支持PC12神经突的形成（28.0±3.0%的细胞）和神经突的生长（14.2µm），与NGF （50 ng/mL）在培养基（29.0±1.3%,14.4µm）中观察到的结果相当。与未受刺激的PC12细胞在ngf固定的PPyPLGA纤维支架上相比，电刺激PC12细胞在ngf固定的纤维上的神经突发育和神经突长度分别提高了18%和17%。因此，结合神经营养和导电活性的亚微米级纤维支架可以作为神经引导导管等有前途的神经组织工程支架。
Engineered scaffolds simultaneously exhibiting multiple cues are highly desirable for neural tissue regeneration. To this end, we developed a neural tissue engineering scaffold that displays submicrometer-scale features, electrical conductivity, and neurotrophic activity. Specifically, electrospun poly(lactic acid-co-glycolic acid) (PLGA) nanofibers were layered with a nanometer thick coating of electrically conducting polypyrrole (PPy) presenting carboxylic groups. Then, nerve growth factor (NGF) was chemically immobilized onto the surface of the fibers. These NGF-immobilized PPy-coated PLGA (NGF-PPyPLGA) fibers supported PC12 neurite formation (28.0±3.0% of the cells) and neurite outgrowth (14.2 µm median length), which were comparable to that observed with NGF (50 ng/mL) in culture medium (29.0±1.3%, 14.4 µm). Electrical stimulation of PC12 cells on NGF-immobilized PPyPLGA fiber scaffolds was found to further improve neurite development and neurite length by 18% and 17%, respectively, compared to unstimulated cells on the NGF-immobilized fibers. Hence, submicrometer-scale fibrous scaffolds that incorporate neurotrophic and electroconducting activities may serve as promising neural tissue engineering scaffolds such as nerve guidance conduits.