Occurrence and characteristics of the mitochondrial permeability transition in plants
Occurrence and characteristics of the mitochondrial permeability transition in plants
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DOI：
10.1074/jbc.m109416200
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发表时间：
2002-01-18
影响因子：
4.8
通讯作者：
Braendle, R
中科院分区：
文献类型：
--
作者：
Arpagaus, S;Rawyler, A;Braendle, R
用光散射、荧光、SDS-聚丙烯酰胺凝胶电泳和免疫印迹技术研究了纯化的马铃薯线粒体对动物线粒体通透性转换的主要效应物的行为。加入Ca 2+诱导磷酸盐依赖性肿胀，如果存在二硫代氨基甲酸，则其完全被环孢菌素A抑制。Mg 2+不能取代Ca 2+，但与之竞争。在溶胀完成后，发生外膜的破坏和几种蛋白质的释放，包括细胞色素c。Ca 2+诱导的溶胀被延迟，其速率降低时，pH值从7.4到6.6。二酰胺、氧化苯胂和亚麻酸可加速反应。然而，在缺乏Ca 2+的情况下，亚麻酸（小于或等于20 μ m）迅速消散琥珀酸驱动的膜电位，而对线粒体体积没有影响。缺氧条件有利于在体外肿胀和伴随释放的细胞色素c和其他蛋白质的pH值依赖性的方式。这些数据表明，经典的线粒体通透性转换也发生在植物中。这可能对我们理解细胞应激和死亡过程具有重要意义。
dThe behavior of purified potato mitochondria toward the main effectors of the animal mitochondrial permeability transition has been studied by light scattering, fluorescence, SDS-polyacrylamide gel electrophoresis, and immunoblotting techniques. The addition of Ca2+ induces a phosphate-dependent swelling that is fully inhibited by cyclosporin A if dithioerythritol is present. Mg2+ cannot be substituted for Ca2+ but competes with it. Disruption of the outer membrane and release of several proteins, including cytochrome c, occur upon completion of swelling. Ca2+-induced swelling is delayed and its rate is decreased when pH is shifted from 7.4 to 6.6. It is accelerated by diamide, phenylarsine oxide, and linolenic acid. In the absence of Ca2+, however, linolenic acid (less than or equal to20 mum) rapidly dissipates the succinate-driven membrane potential while having no effect on mitochondrial volume. Anoxic conditions favor in vitro swelling and the concomitant release of cytochrome c and of other proteins in a pH-dependent way. These data indicate that the classical mitochondrial permeability transition occurs also in plants. This may have important implications for our understanding of cell stress and death processes.