Synergistic Effect of H2O2 and NO2 in Cell Death Induced by Cold Atmospheric He Plasma.
Synergistic Effect of H2O2 and NO2 in Cell Death Induced by Cold Atmospheric He Plasma.
复制标题
H2O2和NO2在寒冷大气中血浆引起的细胞死亡中的协同作用。
DOI：
10.1038/srep29098
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发表时间：
2016-07-01
影响因子：
4.6
通讯作者：
Sousa JS
中科院分区：
文献类型：
--
作者：
Girard PM;Arbabian A;Fleury M;Bauville G;Puech V;Dutreix M;Sousa JS
冷大气压等离子体（CAPP）在过去十年中作为一种新的有前途的抗癌疗法而出现。CAPP的抗肿瘤活性主要是由于活性氧和氮物质（RONS）的传递，但与这种抗癌过程相关的成分的精确测定仍有待完成。在本研究中，使用氦气（He）中产生的微等离子体射流，我们证明了H2 O2，NO2-和NO3-的浓度可以完全解释等离子体激活缓冲液中产生的大部分RONS。研究了这些物种对正常和肿瘤细胞系活力的作用。虽然对H2 O2的敏感度是细胞类型依赖性的，我们表明，H2 O2单独不能解释的毒性，他血浆。事实上，NO2-，而不是NO3-，与H2 O2协同作用，将正常和肿瘤细胞系中的细胞死亡提高到与血浆处理后观察到的水平相似的水平。我们的研究结果表明，等离子体处理的效率在很大程度上取决于确定浓度的H2 O2和NO2−的组合。我们还表明，He等离子体射流与环境空气的相互作用需要在溶液中产生NO2-和NO3-。
Cold atmospheric pressure plasmas (CAPPs) have emerged over the last decade as a new promising therapy to fight cancer. CAPPs’ antitumor activity is primarily due to the delivery of reactive oxygen and nitrogen species (RONS), but the precise determination of the constituents linked to this anticancer process remains to be done. In the present study, using a micro-plasma jet produced in helium (He), we demonstrate that the concentration of H2O2, NO2− and NO3− can fully account for the majority of RONS produced in plasma-activated buffer. The role of these species on the viability of normal and tumour cell lines was investigated. Although the degree of sensitivity to H2O2 is cell-type dependent, we show that H2O2 alone cannot account for the toxicity of He plasma. Indeed, NO2−, but not NO3−, acts in synergy with H2O2 to enhance cell death in normal and tumour cell lines to a level similar to that observed after plasma treatment. Our findings suggest that the efficiency of plasma treatment strongly depends on the combination of H2O2 and NO2− in determined concentrations. We also show that the interaction of the He plasma jet with the ambient air is required to generate NO2− and NO3− in solution.