A histological atlas of the tissues and organs of neotenic and metamorphosed axolotl
A histological atlas of the tissues and organs of neotenic and metamorphosed axolotl
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DOI：
10.1016/j.acthis.2016.07.006
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发表时间：
2016-01-01
影响因子：
2.5
通讯作者：
Keskin, Ilknur
中科院分区：
文献类型：
--
作者：
Demircan, Turan;Ilhan, Ayse Elif;Keskin, Ilknur
Axolotl （Ambystoma Mexicanum）由于其独特的再生能力，在干细胞和再生研究中成为一种有前景的模型。虽然它代表了终身持续的青春期，但甲状腺激素（THs）治疗诱导变态促进了蝾螈在各种研究中的应用。据报道，两栖动物在变态过程中会经历解剖学和组织学的重塑，这种转变对适应陆地环境至关重要。然而，关于美西螈在整个蜕变过程中器官和组织的形态变化，尚无全面的组织学研究。在此，我们揭示了新生期和变质期美西螈组织之间的组织学差异或相似性。为了检查蝾螈器官的结构特征和细胞组织，我们进行了苏木精&伊红、Luxol-Fast蓝、Masson三色、Alcian蓝、Orcein和Weigart染色。在光镜下分析脑、胆囊、心、肠、肝、肺、肌肉、皮肤、脾、胃、尾、舌和血管的染色样品。我们的研究结果有助于验证新获得的功能与尾巴、皮肤、胆囊和脾脏等组织器官结构变化之间的联系。我们相信这一描述工作为更好地理解新生期和变质期蝾螈组织提供了新的见解。(C) 2016 Elsevier GmbH版权所有。
Axolotl (Ambystoma Mexicanum) has been emerging as a promising model in stem cell and regeneration researches due to its exceptional regenerative capacity. Although it represents lifelong lasting neoteny, induction to metamorphosis with thyroid hormones (THs) treatment advances the utilization of Axolotl in various studies. It has been reported that amphibians undergo anatomical and histological remodeling during metamorphosis and this transformation is crucial for adaptation to terrestrial conditions. However, there is no comprehensive histological investigation regarding the morphological alterations of Axolotl organs and tissues throughout the metamorphosis. Here, we reveal the histological differences or resemblances between the neotenic and metamorphic axolotl tissues. In order to examine structural features and cellular organization of Axolotl organs, we performed Hematoxylin & Eosin, Luxol-Fast blue, Masson's trichrome, Alcian blue, Orcein and Weigart's staining. Stained samples from brain, gallbladder, heart, intestine, liver, lung, muscle, skin, spleen, stomach, tail, tongue and vessel were analyzed under the light microscope. Our findings contribute to the validation of the link between newly acquired functions and structural changes of tissues and organs as observed in tail, skin, gallbladder and spleen. We believe that this descriptive work provides new insights for a better histological understanding of both neotenic and metamorphic Axolotl tissues. (C) 2016 Elsevier GmbH. All rights reserved.