Smart-seq2 for sensitive full-length transcriptome profiling in single cells
Smart-seq2 for sensitive full-length transcriptome profiling in single cells
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DOI：
10.1038/nmeth.2639
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发表时间：
2013-11-01
期刊：
影响因子：
48
通讯作者：
Sandberg, Rickard
中科院分区：
文献类型：
--
作者：
Picelli, Simone;Bjorklund, Asa K.;Sandberg, Rickard
单细胞基因表达分析有望表征细胞异质性，但目前的方法在覆盖率、灵敏度或通量上折衷。在这里，我们引入了Smart-seq 2，改进了逆转录，模板转换和预扩增，以增加从单个细胞产生的cDNA文库的产量和长度。与Smart-seq文库相比，Smart-seq 2转录组文库具有改进的检测、覆盖、偏倚和准确性，并且以较低的成本用现成试剂生成。
Single-cell gene expression analyses hold promise for characterizing cellular heterogeneity, but current methods compromise on either the coverage, the sensitivity or the throughput. Here, we introduce Smart-seq2 with improved reverse transcription, template switching and preamplification to increase both yield and length of cDNA libraries generated from individual cells. Smart-seq2 transcriptome libraries have improved detection, coverage, bias and accuracy compared to Smart-seq libraries and are generated with off-the-shelf reagents at lower cost.