Preclinical Evaluation of Ice-Free Cryopreserved Arteries: Structural Integrity and Hemocompatibility
Preclinical Evaluation of Ice-Free Cryopreserved Arteries: Structural Integrity and Hemocompatibility
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DOI：
10.1159/000334544
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发表时间：
2012-01-01
影响因子：
2.7
通讯作者：
Stock, Ulrich A.
中科院分区：
文献类型：
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作者：
Huber, Agnes J. T.;Brockbank, Kelvin G. M.;Stock, Ulrich A.
目的：同种异体动脉移植是治疗人工血管感染的常用方法。通常，使用库存的冷冻保存的动脉;然而，应用于动脉的现有常规冷冻保存技术是昂贵的。相比之下，一种新的无冰冷冻保存技术导致加工，储存和运输方法在技术上更简单，成本可能更低。本研究的目的是确定无冰冷冻保存是否会导致可能妨碍临床使用的组织变化。研究方法：使用形态学（光、扫描电子和激光扫描显微镜）、活力（alamarBlue测定）和血液相容性方法（血细胞粘附、凝血酶/抗凝血酶-III-复合物、多形核纤溶酶-弹性蛋白酶、β-血小板球蛋白和末端补体复合物SC 5 b-9），将常规冷冻冷冻保存的猪动脉与无冰冷冻保存的动脉和未处理的新鲜对照进行比较。结果：无冰冷冻保存和冷冻组织之间没有统计学显著的结构或血液相容性差异。通过激光扫描显微镜测量，未观察到自发荧光（弹性蛋白）或二次谐波产生（胶原蛋白）的定量差异。与新鲜和冷冻组织相比，无冰冷冻保存的动脉中的细胞活力显著降低（p < 0.05）。结论：主动脉保存中冰的形成在组织学、结构或血栓形成方面没有差异，但与排除冰形成的保存方法相比，显著增加了生存能力。降低的细胞活力不应降低体内性能。因此，无冰冷冻保存是一种潜在的安全和成本效益的技术，用于血管移植物的冷冻保存。版权所有（C）2012 S. Karger AG，巴塞尔
Objective: Arterial allografts are routinely employed for reconstruction of infected prosthetic grafts. Usually, banked cryopreserved arteries are used; however, existing conventional freezing cryopreservation techniques applied to arteries are expensive. In contrast, a new ice-free cryopreservation technique results in processing, storage and shipping methods that are technically simpler and potentially less costly. The objective of this study was to determine whether or not ice-free cryopreservation causes tissue changes that might preclude clinical use. Methods: Conventionally frozen cryopreserved porcine arteries were compared with ice-free cryopreserved arteries and untreated fresh controls using morphological (light, scanning electron and laser scanning microscopy), viability (alamarBlue assay) and hemocompatibility methods (blood cell adhesion, thrombin/antithrombin-III-complex, polymorphonuclear neutrophil-elastase, beta-thromboglobulin and terminal complement complex SC5b-9). Results: No statistically significant structural or hemocompatibility differences between ice-free cryopreserved and frozen tissues were detectable. There were no quantitative differences observed for either autofluorescence (elastin) or second harmonic generation (collagen) measured by laser scanning microscopy. Cell viability in ice-free cryopreserved arteries was significantly reduced compared to fresh and frozen tissues (p < 0.05). Conclusions: The formation of ice in aortic artery preservation did not make a difference in histology, structure or thrombogenicity, but significantly increased viability compared with a preservation method that precludes ice formation. Reduced cell viability should not reduce in vivo performance. Therefore, ice-free cryopreservation is a potentially safe and cost-effective technique for the cryopreservation of blood vessel allografts. Copyright (C) 2012 S. Karger AG, Basel