Comparison of the plaque assay and 50% tissue culture infectious dose assay as methods for measuring filovirus infectivity
Comparison of the plaque assay and 50% tissue culture infectious dose assay as methods for measuring filovirus infectivity
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DOI：
10.1016/j.jviromet.2013.05.015
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发表时间：
2013-11-01
影响因子：
3.1
通讯作者：
Olinger, Gene G., Jr.
中科院分区：
文献类型：
--
作者：
Smither, Sophie J.;Lear-Rooney, Calli;Olinger, Gene G., Jr.
对量化丝状病毒感染性的两种常用方法——空斑试验和50%细胞培养感染剂量（TCID50）终点稀释试验进行了比较。使用相同的病毒储备样本同时进行这两种试验，以确定两种试验结果之间的相关性。TCID50试验似乎更敏感，但变异性稍大，而且这些计数方法的数值结果存在10倍差异。讨论了这两种试验的优缺点。这两种方法在丝状病毒研究中都有用且可行，这种比较对于寻求更加团结的丝状病毒研究群体将非常有益。应该在该领域开展进一步的工作以确保丝状病毒研究的一致性。（C）2013由爱思唯尔出版集团出版
Two common methods of quantifying filovirus infectivity, a plaque assay and 50% cell culture infectious dose (TCID50) endpoint dilution assay, were compared. The two assays were performed side by side using the same virus stock sample to determine the correlation between the results of the two assays. The TCID50 assay appeared to be more sensitive but slightly more variable, and there was a tenfold difference in the numerical results of these methods of enumeration. The advantages and disadvantages of both assays are discussed. Both methods are useful and practicable in filovirus research, and this comparison will be hugely beneficial to the filovirus research community as it seeks to become more united. Further work in this area should be performed to ensure consistency in filovirus research. (C) 2013 Published by Elsevier B.V.