Neural engineering to produce in vitro nerve constructs and neurointerface
Neural engineering to produce in vitro nerve constructs and neurointerface
复制标题
DOI：
10.1227/01.neu.0000249197.61280.1d
复制
发表时间：
2007-01-01
期刊：
影响因子：
4.8
通讯作者：
Smith, Douglas H.
中科院分区：
文献类型：
--
作者：
Pfister, Bryan J.;Huang, Jason H.;Smith, Douglas H.
目的：最近，我们的实验室总结了发生在胚胎发育晚期和成年期早期的一种自然形式的轴突生长。在这篇文章中，我们描述了如何利用这种新的神经工程方法来产生神经组织界面，以整合分离的运动和感觉功能以用于外部控制。方法：对于神经系统修复，我们最近开发了一种独特的方法来在体外设计神经组织结构，该结构由横跨两个神经元体细胞群的轴突束组成。为了将电子和神经组织相结合，将电生理信号转换为电子信号，我们设计了一个神经组织接口。结果：我们的神经组织接口由拉伸生长的神经组织组成，一端连接多个电极阵列，使我们能够检测和记录沿着神经传导的实时传出信号，并刺激传入感觉信号。结论：我们的最终目标是开发一种神经控制的假体和一个可以连接到患者思维的神经系统接口，为运动控制和来自多种外部刺激的反馈提供双向信号。
OBJECTIVE: Recently, our laboratory recapitulated a natural form of axon growth that occurs between late embryogenesis and early adulthood. In this article, we describe how this novel neural engineering approach may be used to produce a nervous tissue interface to integrate disconnected motor and sensory functions for external control.METHODS: For nervous system repair, we recently developed a unique method to engineer nervous tissue constructs in vitro consisting of bundles of axons spanning two populations of neuronal somata. To integrate electronics and nervous tissue to transform electrophysiological signals into electronic signals, we have designed a nervous tissue interface.RESULTS: Our nervous tissue interface consists of stretch-grown nervous tissue with one end interfaced with a multiple electrode array, enabling us to detect and record real-time efferent signals conducted down the nerve and stimulate afferent sensory signaling.CONCLUSION: Our ultimate goal is to develop a neurally controlled prosthesis and a nervous system interface that could be linked to the patient's thoughts, providing two-way signaling for motor control and feedback from multiple external stimuli.