GENERATION OF REACTIVE OXYGEN SPECIES, LIPID-PEROXIDATION, AND HUMAN-SPERM FUNCTION
GENERATION OF REACTIVE OXYGEN SPECIES, LIPID-PEROXIDATION, AND HUMAN-SPERM FUNCTION
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DOI：
10.1095/biolreprod41.1.183
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发表时间：
1989-07-01
影响因子：
3.6
通讯作者：
FISHEL, S
中科院分区：
文献类型：
--
作者：
AITKEN, RJ;CLARKSON, JS;FISHEL, S
最近的研究表明，人类精子能够产生活性氧，并且这种活性在精子功能缺陷的情况下显著加速。鉴于脂质过氧化在介导自由基对细胞的损伤中所起的关键作用，我们研究了活性物质产生、脂质过氧化和人类精子功能能力之间的关系。使用丙二醛的生产中存在的亚铁离子启动子作为脂质过氧化的指标，我们已经表明，脂质过氧化显着加速人口的缺陷精子表现出高水平的活性氧产生或在正常细胞刺激产生氧自由基的离子载体，A23187。脂质过氧化的功能后果包括人精子表现出精子卵母细胞融合的能力的剂量依赖性降低，这可以通过包含断链抗氧化剂α-生育酚。低水平的脂质过氧化反应也有轻微的增强作用的活性氧的产生响应离子载体，而不影响稳态活动。在更高水平的脂质过氧化，无论是基础水平的活性氧产生和A23187的反应显着减少。相反，脂质过氧化对人精子通过可再次被α-半胱氨酸逆转的机制结合同源和异源透明带的能力具有高度显著的增强作用。生育酚。
Recent studies have demonstrated that human spermatozoa are capable of generating reactive oxygen species and that this activity is significantly accelerated in cases of defective sperm function. In view of the pivotal role played by lipid peroxidation in mediating free radical damage to cells, we have examined the relationships between reactive species production, lipid peroxidation, and the functional competence of human spermatozoa. Using malondialdehyde production in the presence of ferrous ion promoter as an index of lipid peroxidation, we have shown that lipid peroxidation is significantly accelerated in populations of defective spermatozoa exhibiting high levels of reactive oxygen species production or in normal cells stimulated to produce oxygen radicals by the ionophore, A23187. The functional consequences of lipid peroxidation included a dose-dependent reduction in the ability of human spermatozoa to exhibit sperm oocyte-fusion, which could be reversed by the inclusion of a chain-breaking antioxidant, .alpha.-tocopherol. Low levels of lipid peroxidation also had a slight enhancing effect on the generation of reactive oxygen species in response to ionophore, without influencing the steady-state activity. At higher levels of lipid peroxidation, both the basal level of reactive oxygen species production and the response to A23187 were significnatly diminished. In contrast, lipid peroxidation had a highly significant, enhancing effect on the ability of human spermatozoa to bind to both homologous and heterologous zonae pellucidae via mechanisms that could again be reversed by .alpha.-tocopherol.