Changes of abundance and diversity of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in three nitrifying bioreactors with different ammonia concentrations
Changes of abundance and diversity of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in three nitrifying bioreactors with different ammonia concentrations
复制标题
不同氨浓度的三个硝化生物反应器中氨氧化古菌（AOA）和细菌（AOB）的丰度和多样性变化
DOI：
10.1080/19443994.2015.1123196
复制
发表时间：
2016-01-01
影响因子：
1.1
通讯作者：
Pan, Kailing
中科院分区：
文献类型：
--
作者：
Gao, Jingfeng;Fan, Xiaoyan;Pan, Kailing
与氨氧化细菌（AOB）不同，废水处理系统中氨浓度对氨氧化古菌（AOA）的影响研究较少。本研究利用三个序批式反应器对含AOA和AOB的污泥进行了富集采用PCR、克隆、测序和实时荧光定量PCR（qPCR）技术，研究了不同氨氮浓度（14、56和140 mg/L）下，AOA和AOB（分别命名为R1、R2和R3）的多样性、丰度以及对氨氮氧化的贡献。克隆结果表明，在140 d的运行过程中，反应器中只有一个优势AOA物种（亚硝化球菌簇）。然而，AOB社区之间的反应器变化显着。经过140 d的富集，R1和R2中的优势AOB菌群--亚硝化单胞菌（Nitrosomonasuericum）也占优势，而R3中的优势菌群--欧洲亚硝化单胞菌（Nitrosomonaseuropaea）也得到富集。3种氨氮水平下AOB的多样性均高于AOA。高氨氮浓度下（R3）氨氧化细菌多样性低于低氨氮浓度下（R1和R2）。QPCR结果表明，在运行期间，不同氨氮浓度下AOA丰度几乎没有变化。运行140 d后，AOB/AOA比值由接种污泥的3.68 × 10 ~（2）增加到4.90 × 10 ~（3）、1.25 × 10 ~（5）和3.77 × 10 ~（5）。研究表明，在高氨氮浓度环境中，AOB比AOA更具竞争力，在氨氧化过程中可能比AOA发挥更重要的作用。
Different from ammonia-oxidizing bacteria (AOB), the influence of ammonia levels on ammonia-oxidizing archaea (AOA) in wastewater treatment systems was poorly understood. In this study, sludge contained AOA and AOB was enriched in three sequencing batch reactors (named as R1, R2, and R3) under different ammonia levels (14, 56, and 140mg/L) using polymerase chain reaction (PCR), cloning, sequencing, and quantitative real-time PCR (qPCR) to evaluate the effect of ammonia levels on the diversity, abundance, and contribution of AOA and AOB to ammonia oxidation. Cloning results showed that there was only one dominant AOA species (Nitrososphaera cluster) in the reactors during 140-d operation. However, AOB communities varied significantly among the reactors. After 140-d enrichment, Nitrosomonas ureae cluster, the dominant AOB cluster in seed sludge, was also dominant in R1 and R2, while Nitrosomonas europaea cluster was enriched and dominated in R3. Diversity of AOB was higher than AOA under the three ammonia levels. Diversity of AOB under high ammonia concentration (in R3) was lower than that under low ammonia concentrations (in R1 and R2). QPCR results revealed that AOA abundance was almost unchanged under different ammonia levels during operational period. The ratio of AOB to AOA increased from 3.68x10(2) in seed sludge to 4.90x10(3), 1.25x10(5), and 3.77x10(5) in three reactors after 140-d running. This study suggested that AOB was much more competitive than AOA in high ammonia level environments and probably played a more important role than AOA in ammonia oxidation.