A completely biological tissue‐engineered human blood vessel
A completely biological tissue‐engineered human blood vessel
复制标题
DOI：
10.1096/fsb2fasebj.12.1.47
复制
发表时间：
1998-01
期刊：
影响因子：
--
通讯作者：
N. L’Heureux;Stéphanie Pâquet;R. Labbé;L. Germain;F. Auger
中科院分区：
文献类型：
--
作者：
N. L’Heureux;Stéphanie Pâquet;R. Labbé;L. Germain;F. Auger
受到机械挑战的组织工程器官，如血管，传统上依赖合成或修饰的生物材料作为结构支撑。在这份报告中，我们提出了一种新的方法来制造组织工程血管(TEBV)，该方法完全基于培养的人类细胞，即不使用任何合成或外源生物材料。用抗坏血酸培养的人血管平滑肌细胞(SMC)产生了一层粘连的细胞膜。该薄片被放置在管状支撑物周围以产生容器的介质。一张类似的人类成纤维细胞被包裹在介质周围，以提供外膜。成熟后，去除小管支架，在管腔内种植内皮细胞。这种TEBV具有定义明确的三层组织和大量的细胞外基质蛋白，包括弹性蛋白。在这种环境下，SMC重新表达了结蛋白，这是一种已知在标准培养条件下丢失的分化标记。体外培养的内皮细胞表达von Willebrand因子，掺入乙酰化的低密度脂蛋白，产生PGI2，强烈抑制血小板黏附。整个容器的抗爆强度超过2000毫米汞。这是第一个显示出与人类血管相当的爆裂强度的完全生物TEBV。在犬模型上的短期移植实验显示出良好的可控性和可缝合性。综上所述，这些结果表明，这项新技术可以产生完全生物的血管，满足移植的基本要求：高破裂强度、积极的外科处理和功能内皮。-L的Heureux，N.，Paquet，S.，Labbe，R.，Germain，L.，Auger，F.A.A完全生物组织工程人类血管。FASE B J.12，47-56(1998)
Mechanically challenged tissue‐engineered organs, such as blood vessels, traditionally relied on synthetic or modified biological materials for structural support. In this report, we present a novel approach to tissue‐engineered blood vessel (TEBV) production that is based exclusively on the use of cultured human cells, i.e., without any synthetic or exogenous biomaterials. Human vascular smooth muscle cells (SMC) cultured with ascorbic acid produced a cohesive cellular sheet. This sheet was placed around a tubular support to produce the media of the vessel. A similar sheet of human fibroblasts was wrapped around the media to provide the adventitia. After maturation, the tubular support was removed and endothelial cells were seeded in the lumen. This TEBV featured a well‐defined, three‐layered organization and numerous extracellular matrix proteins, including elastin. In this environment, SMC reexpressed desmin, a differentiation marker known to be lost under standard culture conditions. The endothelium expressed von Willebrand factor, incorporated acetylated LDL, produced PGI2, and strongly inhibited platelet adhesion in vitro. The complete vessel had a burst strength over 2000 mmHg. This is the first completely biological TEBV to display a burst strength comparable to that of human vessels. Short‐term grafting experiment in a canine model demonstrated good handling and suturability characteristics. Taken together, these results suggest that this novel technique can produce completely biological vessels fulfilling the fundamental requirements for grafting: high burst strength, positive surgical handling, and a functional endothelium.—L'Heureux, N., Paquet, S., Labbe, R., Germain, L., Auger, F. A. A completely biological tissue‐engineered human blood vessel. FASEB J. 12, 47–56 (1998)