Patterns of Variation in Levels of Hsp70 in Natural Rocky Shore Populations from Microscales to Mesoscales1
Patterns of Variation in Levels of Hsp70 in Natural Rocky Shore Populations from Microscales to Mesoscales1
复制标题
天然岩石海岸种群中 Hsp70 水平从微尺度到中尺度的变化模式1
DOI：
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发表时间：
2002
影响因子：
2.6
通讯作者：
B. Menge
中科院分区：
文献类型：
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作者：
Patricia M. Halpin;C. Sorte;G. Hofmann;B. Menge
摘要将生物体对热应激的反应与群落结构模式联系起来的一个重要步骤是确定在多大范围内表现出可识别的变化水平。生物可能对其做出反应的温度信号在许多空间尺度上都不同，包括微生境、潮汐高度、地点和纬度。一些研究已经将潮间带热休克反应(HSR)的生理评估作为一种工具来研究自然界中的HSR，并研究HSR分子作为种群或群落水平指标的效用。最常见的是对Hsp70亚型家族总库的免疫检测。在这里，我们介绍了潮间带生物从小生境到中尺度的HSP70水平的数据。我们的数据和以前发表的工作表明，Hsp70水平在所研究的所有尺度上都不同，类似于其他感兴趣的物理和生物变量。这证明了Hsp70检测作为现场生物学家的分子工具的潜在效用，以及在寻找潜在的生物指示剂分子时在评估变异规模时必须注意的事项。
Abstract An important step in connecting the organismal response to thermal stress to patterns of community structure is determining at what scale discernable levels of variation are manifested. The temperature signal to which organisms may potentially respond varies at many spatial scales including microhabitat, tidal height, site and latitude. A number of studies have taken physiological assessment of the heat shock response (HSR) into the intertidal both as a tool for examining the HSR in nature and for examining the utility of HSR molecules as population or community level indicators. Most commonly, immunodetection of the total pool of the Hsp70 family of isoforms is used. Here we present data on levels of Hsp70 in intertidal organisms from microhabitat to the mesoscale. Our data and previously published work show that Hsp70 levels vary at all scales examined, similar to other physical and biological variables of interest. This demonstrates both the potential utility of Hsp70 detection as a molecular tool for field biologists and to the care that must be taken in assessing scale of variation when looking for potential bioindicator molecules.