A novel low-density lipoprotein receptor-related protein with type II membrane protein-like structure is abundant in heart.
A novel low-density lipoprotein receptor-related protein with type II membrane protein-like structure is abundant in heart.
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心脏中富含一种新型低密度脂蛋白受体相关蛋白，具有II型膜蛋白样结构。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
Tokuo T. Yamamoto
中科院分区：
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作者：
Yasuhiro Tomita;Dong;K. Magoori;T. Fujino;Tokuo T. Yamamoto
我们在此报道了低密度脂蛋白受体 (LDLR) 家族新成员的鉴定，称为 LDLR 相关蛋白 4 (LRP4)。鼠 LRP4 cDNA 编码 1113 个氨基酸的 II 型膜样蛋白，在两个簇中具有 8 个配体结合重复序列。对几种不同生物体的基因组 DNA 进行 Southern 印迹分析表明，在缺乏编码载脂蛋白 E 的基因的鸡中存在 LRP4 同源物，载脂蛋白 E 可以被 LDLR 的配体结合重复序列识别。 LRP4 转录本几乎只在小鼠和人类的心脏中检测到。尽管存在配体结合重复序列，但转染LRP4的COS细胞并未表现出β-迁移极低密度脂蛋白的表面结合，这表明LRP4在脂蛋白代谢以外的途径中发挥作用。
We report herein the identification of a novel member of the low-density lipoprotein receptor (LDLR) family termed LDLR-related protein 4 (LRP4). Murine LRP4 cDNA encodes a 1113-amino-acid type II membrane-like protein with eight ligand-binding repeats in two clusters. Southern blot analysis of genomic DNA from several different organisms suggests the presence of LRP4 homologues in chicken lacking the gene encoding apolipoprotein E, which is recognized by the ligand-binding repeats of LDLR. LRP4 transcripts were detected almost exclusively in heart in mouse and humans. Despite the presence of the ligand-binding repeats, COS cells transfected with LRP4 did not show surface-binding of beta-migrating very-low-density lipoprotein, suggesting that LRP4 plays a role in a pathway other than lipoprotein metabolism.