Effects of signaling molecules, protein phosphatase inhibitors and blast pathogen (Magnaporthe grisea) on the mRNA level of a rice (Oryza sativa L.) phospholipid hydroperoxide glutathione peroxidase (OsPHGPX) gene in seedling leaves
Effects of signaling molecules, protein phosphatase inhibitors and blast pathogen (Magnaporthe grisea) on the mRNA level of a rice (Oryza sativa L.) phospholipid hydroperoxide glutathione peroxidase (OsPHGPX) gene in seedling leaves
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DOI：
10.1016/s0378-1119(01)00854-x
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发表时间：
2002-01-23
期刊：
影响因子：
3.5
通讯作者：
Agrawal, VP
中科院分区：
文献类型：
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作者：
Agrawal, GK;Rakwal, R;Agrawal, VP
以水稻自卫反应为特定关注点，研究了全球信号分子茉莉酸（JA）、水杨酸（SA）、脱落酸（ABA）和乙烯（使用乙烯发生剂乙烯利）以及蛋白磷酸酶（PP）抑制剂斑蝥素和草藻灭对水稻幼苗叶片中一个水稻磷脂氢过氧化物谷胱甘肽过氧化物酶（OsPHGPX）基因表达的影响。我们首次提供证据表明这些信号分子和PP抑制剂对OsPHGPX mRNA的积累有强烈的上调作用，这有力地暗示了它在防御/应激中的潜在作用。OsPHGPX基因也表现出微弱的组成型表达以及对切割的响应性。这些诱导受光信号影响，并且未显示出对新合成蛋白质因子的需求。发现这些信号分子之间，特别是JA、SA、ABA和激动素之间在调节OsPHGPX表达方面存在潜在的相互作用。稻瘟病菌（Magnaporthe grisea）也引发了叶片中OsPHGPX mRNA的积累。这是在水稻（以及植物中）的第一份系统性报告，证明了OsPHGPX基因可由多种与防御/应激相关的刺激诱导（和表达），以及蛋白磷酸酶对激酶信号级联的调节作用。（C）2002 Elsevier Science B.V. 保留所有权利。
With a specific focus on rice self-defense response(s), the effects of global signaling molecules, jasmonic acid (JA), salicylic acid (SA). abscisic acid (ABA), and ethylene (using the ethylene generator, ethephon and protein phosphatase (PP) inhibitors. cantharidin and endothall on expression of a rice phospholipid hydroperoxide glutathione peroxidase (OsPHGPX) gene in rice seedling leaves were investigated. We provide first evidence for a potent up-regulation of the OsPHGPX mRNA accumulation by these signaling molecules and PP inhibitors that strongly suggest its potential role in defense/stress. The OsPHGPX gene also showed a weak constitutive expression and responsiveness to Cut. These inductions were influenced by light signal(s), and did not show a requirement for de novo synthesized protein factor(s). A potential interaction amongst these signaling molecules, especially JA, SA, ABA and kinetin, in modulating the OsPHGPX expression was found. The blast pathogen, Magnaporthe grisea also elicited the accumulation of OsPHGPX mRNA in leaves. This is a first systematic report in rice (and in plants) demonstrating the inducible nature (and expression) of the OsPHGPX gene by a variety of defense/stress-related stimuli, and modulation by the PPs of the kinase-signaling cascade(s). (C) 2002 Elsevier Science B.V. All rights reserved.