Evaluation of intraspecies genetic variation within the 16S rRNA gene of Mycoplasma hominis and detection by polymerase chain reaction
Evaluation of intraspecies genetic variation within the 16S rRNA gene of Mycoplasma hominis and detection by polymerase chain reaction
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人型支原体16S rRNA基因种内遗传变异评价及聚合酶链式反应检测
DOI：
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发表时间：
1993
影响因子：
9.4
通讯作者：
Gail H. Cassell
中科院分区：
文献类型：
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作者：
Alain Blanchard;Antonio Yáñez;Kevin Dybvig;H. Watson;G. Griffiths;Gail H. Cassell
人型支原体是一种异质种，DNA-DNA杂交值范围为51%至100%。我们在这里报告的16 S rRNA基因的测序菌株（183），大大不同于该物种的模式菌株（PG 21）。比较两株菌的16 SrDNA序列发现，两株菌的16 SrDNA序列差异不大，表明两株菌属于同一种rRNA种复合体。利用这些核苷酸序列数据，我们建立了一种快速检测M.用聚合酶链反应检测人的DNA。用参考菌株和临床分离株进行检测时，该方法显示出敏感性和特异性。
Mycoplasma hominis is a heterogeneous species with DNA-DNA hybridization values ranging from 51 to 100%. We report here the sequencing of the 16S rRNA gene of a strain (183) that greatly differs from the type strain (PG21) of this species. Comparison of 16S rDNA sequences from these two strains showed limited differences, indicating that the two strains belong to the same rRNA species complex. Using these nucleotide sequence data, we established a rapid method for the detection of M. hominis by using polymerase chain reaction. This method was shown to be sensitive and specific when tested with reference strains and clinical isolates.