RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components
RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components
复制标题
DOI：
10.1101/gad.1226604
复制
发表时间：
2004-10-15
影响因子：
10.5
通讯作者：
Baserga, SJ
中科院分区：
文献类型：
--
作者：
Gallagher, JEG;Dunbar, DA;Baserga, SJ
在大分子生物合成过程中，连续的事件通常是协调的。小核糖体亚基（SSU）加工体是一种大的核糖核蛋白（RNP），用于加工核糖体小亚基RNA （18S）的前体。我们发现SSU过程蛋白的一个亚复合物，t-Utps，也是酵母体内最佳rRNA转录所必需的。t-Utps与核糖体染色质（r-染色质）相关，它们存在于没有U3 snoRNA的复合体中。转录既不需要亚复合物的形成，也不需要它的r-染色质结合。t-Utps与前18s rnas相关，独立于U3 snoRNA的存在。因此，这种联系可能代表了SSU过程形成的早期步骤。我们的研究结果表明，rRNA转录和前rRNA加工是通过SSU加工体的特定成分协调的。
Sequential events in macromolecular biosynthesis are often elegantly coordinated. The small ribosomal subunit (SSU) processome is a large ribonucleoprotein (RNP) required for processing of precursors to the small subunit RNA, the 18S, of the ribosome. We have found that a subcomplex of SSU processome proteins, the t-Utps, is also required for optimal rRNA transcription in vivo in the yeast Saccharomyces cerevisiae. The t-Utps are ribosomal chromatin (r-chromatin)-associated, and they exist in a complex in the absence of the U3 snoRNA. Transcription is required neither for the formation of the subcomplex nor for its r-chromatin association. The t-Utps are associated with the pre-18S rRNAs independent of the presence of the U3 snoRNA. This association may thus represent an early step in the formation of the SSU processome. Our results indicate that rRNA transcription and pre-rRNA processing are coordinated via specific components of the SSU processome.