A pilot study to investigate the histomorphometric changes of murine maxillary bone around the site of mini-screw insertion in regenerated bone induced by anabolic reagents
A pilot study to investigate the histomorphometric changes of murine maxillary bone around the site of mini-screw insertion in regenerated bone induced by anabolic reagents
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DOI：
10.1093/ejo/cjaa018
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发表时间：
2021-02-01
影响因子：
2.6
通讯作者：
Ono, Takashi
中科院分区：
文献类型：
--
作者：
Keo, Preksa;Matsumoto, Yoshiro;Ono, Takashi
目的：本研究的目的是调查使用合成代谢肽和骨形态发生蛋白 2 (BMP-2) 的合成代谢注射方法诱导的再生骨中微型螺钉插入部位周围的组织形态变化。方法：使用 27 只 8 周龄的 C57BL/6,1 雄性小鼠。一些小鼠在上颌第一磨牙前面接受了粘膜下联合注射合成代谢肽和 BMP-2。注射后4周进行螺钉插入。所有小鼠在第 8 周处死之前每周进行一次体内微焦 X 射线计算机断层扫描 (mu CT) 分析。使用未脱钙切片中的荧光标记评估骨形成活性。包括螺钉插入在内的分析是在螺钉插入部位前面的额面进行的。结果：重建的 mu CT 图像显示，到第 8 周，共注射合成代谢试剂可导致注射诱导增厚的骨的骨矿物质密度 (BMD) 逐渐增加。放射学和组织形态测量分析均表明，螺钉插入对 BMD 或诱导骨的骨形成活性没有任何有害影响。此外，注射合成代谢试剂还导致注射部位下方上颌骨的 BMD 增加。结论：我们的组织形态计量学分析表明，进行此类合成代谢注射以增厚骨可以刺激基底骨以及诱导骨中的骨形成。即使在随后将螺钉插入诱导骨的部位后，也可以获得类似的骨形成增强。
Objective: The objective of this study was to investigate the histomorphometric changes around the site of mini-screw insertion in the regenerated bone which was induced by an anabolic-injection method using both anabolic peptide and bone morphogenetic protein 2 (BMP-2).Methods: Twenty-seven eight-week-old C57BL/6,1 male mice were used. Some mice received submucosal co-injections of anabolic peptide and BMP-2 just in front of the maxillary first molar. Screw insertion was then performed 4 weeks after injection. All mice underwent a weekly in vivo micro-focal X-ray computed tomography (mu CT) analysis before being sacrificed at week 8. The bone formation activity was evaluated using fluorescent labelling in the undecalcified sections. The analyses, including screw insertion, were performed in the frontal plane, in front of the site of screw insertion.Results: Reconstructed mu CT images revealed that the co-injection of anabolic reagents could lead to a gradual increase in the bone mineral density (BMD) of the injection-induced thickened bone by week 8. Both radiological and histomorphometric analyses indicated that screw insertion did not have any deleterious effects on either the BMD or the bone formation activity of the induced bone. Furthermore, the injection of anabolic reagents also led to an increase in the BMD of the underlying maxillary bone at the injection site.Conclusion: Our histomorphometric analyses suggest that performing such anabolic injection to thicken bone could stimulate bone formation in the basal bone as well as in the induced bone. Similar augmentation of bone formation could be obtained even after subsequent screw insertion at the site of the induced bone.