MONOCLONAL-ANTIBODY ANALYSIS OF NEUTRALIZATION AND ANTIBODY-DEPENDENT ENHANCEMENT OF FELINE INFECTIOUS PERITONITIS VIRUS
MONOCLONAL-ANTIBODY ANALYSIS OF NEUTRALIZATION AND ANTIBODY-DEPENDENT ENHANCEMENT OF FELINE INFECTIOUS PERITONITIS VIRUS
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DOI：
10.1128/jvi.66.11.6695-6705.1992
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发表时间：
1992-11-01
影响因子：
5.4
通讯作者：
SCOTT, FW
中科院分区：
文献类型：
--
作者：
CORAPI, WV;OLSEN, CW;SCOTT, FW
根据蛋白质特异性、免疫球蛋白亚类、病毒中和性、与不同冠状病毒的反应性以及体外诱导FIPV感染的抗体依赖性增强（ADE）的能力，对54株抗猫传染性腹膜炎病毒（FIPV）的单克隆抗体（MAbs）进行了表征。这些单克隆抗体对FIPV的三种结构蛋白中的一种具有特异性。总共47个MAbs对205-kDa刺突蛋白（S）具有特异性，3个MAbs对45-kDa核衣壳蛋白（N）具有特异性，4个MAbs对26-至28-kDa膜蛋白（M）具有特异性。S-特异性单克隆抗体与FIPV、猫肠道冠状病毒、犬冠状病毒和猪传染性胃肠炎病毒株表现出不同程度的交叉反应性。19种S特异性单克隆抗体中和了FIPV。共15个中和单抗诱导ADE，除1个外，其余均为免疫球蛋白G2 a亚类。其余4种不诱导ADE的中和MAb属于免疫球蛋白G1亚类。两种S特异性单克隆抗体诱导ADE，但非中和。N-或M-特异性单克隆抗体均不能中和或诱导ADE。根据单克隆抗体与FIPV和相关冠状病毒的反应模式，得出结论，S.在大多数情况下，中和单克隆抗体能够诱导ADE，证明中和和增强之间的直接关系。诱导ADE和不诱导ADE的中和MAb之间免疫球蛋白亚类的差异表明，可能存在能够介导ADE的免疫球蛋白亚类的限制。
Fifty-four monoclonal antibodies (MAbs) to feline infectious peritonitis virus (FIPV) were characterized according to protein specificity, immunoglobulin subclass, virus neutralization, reactivity with different coronaviruses, and ability to induce antibody-dependent enhancement (ADE) of FIPV infection in vitro. The MAbs were found to be specific for one of three structural proteins of FIPV. A total of 47 MAbs were specific for the 205-kDa spike protein (S), 3 MAbs were specific for the 45-kDa nucleocapsid protein (N), and 4 MAbs were specific for the 26- to 28-kDa membrane protein (M). The S-specific MAbs showed various degrees of cross-reactivity with strains of FIPV, feline enteric coronavirus, canine coronavirus, and porcine transmissible gastroenteritis virus. Nineteen S-specific MAbs neutralized FIPV. A total of 15 of the neutralizing MAbs induced ADE, and all but 1 were of the immunoglobulin G2a subclass. The remaining four neutralizing MAbs that did not induce ADE were of the immunoglobulin G1 subclass. Two S-specific MAbs induced ADE but were nonneutralizing. None of the N- or M-specific MAbs was neutralizing or induced ADE. On the basis of the reactivity patterns of the MAbs with FIPV and related coronaviruses, it was concluded that there is a minimum of five neutralizing sites on S. In most instances, neutralizing MAbs were able to induce ADE, demonstrating a direct relationship between neutralization and enhancement. The difference in immunoglobulin subclass between neutralizing MAbs that induced ADE and those that did not induce ADE suggests that there may be a restriction in the immunoglobulin subclasses capable of mediating ADE.