RHS2, A POU DOMAIN-CONTAINING GENE, AND ITS EXPRESSION IN DEVELOPING AND ADULT-RAT
RHS2, A POU DOMAIN-CONTAINING GENE, AND ITS EXPRESSION IN DEVELOPING AND ADULT-RAT
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DOI：
10.1073/pnas.89.8.3285
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发表时间：
1992-04-15
影响因子：
11.1
通讯作者：
YOUNG, WS
中科院分区：
文献类型：
--
作者：
LEMOINE, C;YOUNG, WS
中枢神经系统内的基因表达受到 DNA 结合蛋白复杂相互作用的调节，其中包括含有 POU 结构域的蛋白，它们与同源盒蛋白关系较远。这些含有 POU 结构域的蛋白质与中枢神经系统内转录和复制的控制有关。我们使用简并引物和 PCR 从下丘脑 RNA 中分离出另一个包含 POU 结构域的 cDNA，RHS2。通过使用在固体培养基上生长的下丘脑 cDNA 文库的系列稀释液来分离推定的全长 cDNA。 III 类 POU 家族的这一成员在从胚胎第 11.5 天到成年期的大鼠中表达，在大脑中尤其突出。我们进行了双标记杂交组织化学分析，确定 RHS2 与尾壳核中等大小神经元中的多种神经肽共表达，以及下丘脑室旁核和视上核中的强啡肽共表达。尾壳核中 RHS2 的表达通过消除其黑质纹状体多巴胺能神经支配而增加。
Gene expression within the central nervous system is regulated by complex interactions of DNA-binding proteins, among which are the POU domain-containing proteins, which are distantly related to homeobox proteins. These POU domain-containing proteins have been implicated in control of transcription and replication within the central nervous system. We used degenerate primers with the PCR to isolate another POU domain-containing cDNA, RHS2, from hypothalamic RNA. Isolation of a putative full-length cDNA was accomplished by using serial dilutions of a hypothalamic cDNA library grown on solid medium. This member of the class III POU family is expressed in rats from embryonic day 11.5 into adulthood, being especially prominent in the brain. We performed double-labeling hybridization histochemistry and determined that RHS2 is coexpressed with a variety of neuropeptides in medium-sized neurons in the caudate putamen and with dynorphin in the paraventricular and supraoptic nuclei of the hypothalamus. Expression of RHS2 in the caudate putamen was increased by elimination of its nigrostriatal dopaminergic innervation.